Anti-Mouse CD11b Monoclonal Antibody (Biotin) for Flow Cytometry - P05555

Core Product Specifications and Parameters

Biological Background: Integrin alpha-M (CD11b) Function and Localization

• Integrin alpha-M (CD11b/ITGAM) is the alpha subunit of the leukocyte integrin Mac-1 (αMβ2, CD11b/CD18), a cell surface receptor essential for innate immunity.
• Core functions include mediating adhesion of monocytes, macrophages, and granulocytes, and acting as the receptor for the complement fragment iC3b (CR3) to promote phagocytosis of opsonized pathogens.
• ITGAM/ITGB2 also binds fibrinogen, factor X, and ICAM1, facilitating leukocyte-endothelial interactions during inflammation.
• The protein is predominantly expressed on the surface of myeloid cells, including monocytes, granulocytes, peritoneal mast cells, and microglia, and localizes to the cell membrane and membrane rafts.
• In microglia, ITGAM partners with TYROBP/DAP12 to regulate superoxide production, contributing to developmental neuronal apoptosis (PubMed:18685038). Related references: PMID:18685038
• Post-translational modifications include disulfide bonds, glycosylation, and calcium/metal-binding, and the protein undergoes alternative splicing.
• The mature protein has a predicted molecular weight of approximately 127 kDa and contains extracellular repeats, a single-pass transmembrane domain, and a short cytoplasmic tail.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the extracellular I-domain of mouse CD11b (amino acids 143–337), a region critical for ligand binding. Consider using this domain for blocking studies.
• This biotinylated monoclonal antibody is optimized for direct flow cytometry (FC). For multi-color panels, pair with compatible fluorophore-streptavidin conjugates and titrate to optimal signal-to-noise.
• As CD11b expression varies with cell activation state, include appropriate isotype controls and validate in your specific mouse sample system (e.g., blood, spleen, or bone marrow cells).
• For applications beyond FC (e.g., IHC, IF, or functional assays), in-house validation is recommended, as this product is supplied for flow cytometry only.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to validated IVD raw materials, including biotin-conjugated antibodies like this anti-mouse CD11b clone. From monoclonal and polyclonal antibody development to bulk ancillary reagents and rare-target sourcing, we support every stage of immunoassay development and in vitro diagnostics. Our comprehensive portfolio ensures you can procure the precise reagents needed for your myeloid cell and inflammation research, all from a single, reliable partner.