Anti-Mouse CD11a Monoclonal Antibody for Flow Cytometry - P24063

Core Product Specifications and Parameters

Biological Background: CD11a (Integrin alpha-L) Function and Localization

• Integrin alpha-L (CD11a) pairs with integrin beta-2 (ITGB2) to form the heterodimeric receptor LFA-1, a key adhesion molecule on leukocytes.
• LFA-1 mediates leukocyte adhesion and transmigration by binding to ICAM1, ICAM2, ICAM3, and ICAM4, essential for immune cell trafficking and inflammatory responses (PubMed:2051027). Related references: PMID:2051027
• It is involved in T-cell and neutrophil migration, cytotoxic T-cell killing, and antibody-dependent cellular cytotoxicity, playing a central role in adaptive and innate immunity (PubMed:16234355, PubMed:24158516). Related references: PMID:16234355 PMID:24158516
• CD11a contributes to natural killer cell cytotoxicity and also serves as a receptor for the secreted form of ubiquitin-like protein ISG15, linking innate antiviral responses (PubMed:29100055). Related references: PMID:29100055
• LFA-1 is required for the generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis (PubMed:25108025). Related references: PMID:25108025
• At the immunological synapse, LFA-1 acts as a platform translating TCR engagement and ICAM1 density into graded adhesion, facilitating T-cell activation.
• Subcellular localization includes the cell membrane and membrane rafts, with tissue-specific expression restricted to leukocytes.
• Post-translational modifications include phosphorylation and glycosylation; the protein contains disulfide bonds, calcium and magnesium binding sites, and a transmembrane helix.

Experimental Guidance and Technical Tips

• This antibody is conjugated to , a far-red fluorescent dye with excitation/emission peaks ~590/617 nm. Ensure your flow cytometer is equipped with appropriate lasers and filters (e.g., 561 nm or 594 nm excitation).
• Standard flow cytometry protocols recommend using isotype-matched control antibodies and proper Fc receptor blocking to minimize non-specific binding.
• For mouse leukocyte analysis, typical staining conditions range from 0.5–5 µg per million cells, but optimal titers should be determined empirically in your specific assay system.
• Note that this antibody is reported to recognize mouse CD11a; cross-reactivity with other species has not been evaluated.
• The immunogen corresponds to amino acids 149–340 (extracellular domain) of mouse CD11a, which may cover functionally relevant epitopes involved in ligand binding or activation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies diagnostic manufacturers and research laboratories with comprehensive IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. For CD11a-focused studies in immunology and inflammation, CamelBio offers reliable procurement solutions to accelerate your assay development. Partner with CamelBio to secure critical reagents from concept to clinic.