Anti-Mouse CD117/c-Kit Rabbit Monoclonal Antibody for FC - P05532

Core Product Specifications and Parameters

Biological Background: CD117/c-Kit Function and Localization

• The KIT gene encodes a receptor tyrosine kinase, also known as mast/stem cell growth factor receptor (SCFR) or CD117, and acts as the receptor for stem cell factor (SCF/KITLG).
• Upon ligand binding, KIT activates multiple signaling cascades, including the PI3K-AKT, RAS-MAPK, and JAK-STAT pathways, governing cell survival, proliferation, differentiation, and migration.
• KIT is essential for hematopoiesis, melanogenesis, gametogenesis, and mast cell function, and its signaling is tightly regulated by internalization and degradation.
• The receptor is a single-pass transmembrane protein localized primarily at the cell membrane but can also be found in the cytoplasm.
• Multiple alternatively spliced isoforms exist, with isoform 1 widely expressed in fetal liver and bone marrow, while isoform 3 is enriched in hematopoietic stem cells.
• Post-translational modifications include autophosphorylation at multiple tyrosine residues, N-linked glycosylation, and ubiquitination, which modulate its activity and turnover.
• As a proto-oncogene, gain-of-function mutations in KIT are implicated in various cancers, making it a therapeutic target.

Experimental Guidance and Technical Tips

• The immunogen spanning the extracellular domain (aa 25-523) suggests suitability for detection of cell surface CD117/c-Kit by flow cytometry; confirm antibody binding in your relevant mouse hematopoietic or stem cell populations.
• When using this antibody for flow cytometry, consider appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding.
• Multiple reported molecular weights (22 kDa, 108 kDa, 109 kDa) correspond to different isoforms or cleavage products; if performing western blot, validate the expected band in your sample system, noting that the supplier has validated the antibody for FC only.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to high-quality IVD raw materials, including this -conjugated rabbit monoclonal anti-mouse CD117 antibody validated for flow cytometry. As a one-stop partner, we offer a comprehensive portfolio covering optimized monoclonal/polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and rare target sourcing support, enabling seamless progression from concept to clinic.