Anti-Mouse CD112/Nectin-2 Monoclonal Antibody (ABflo 488) for FC - P32507

Core Product Specifications and Parameters

Biological Background: Nectin-2 Function and Localization

• Nectin-2 (CD112, PVRL2, herpesvirus entry mediator B) is a cell adhesion protein and member of the nectin family, which belongs to the immunoglobulin superfamily.
• Acts as a modulator of T-cell signaling, capable of either costimulating or coinhibiting T-cell function depending on the interacting receptor.
• Upon binding to CD226, Nectin-2 stimulates T-cell proliferation and production of cytokines such as IL2, IL5, IL10, IL13, and IFNG.
• Interaction with PVRIG results in inhibition of T-cell proliferation; these receptor interactions are competitive.
• Localized to the cell membrane and expressed in tissues including brain, spinal cord, spleen, kidney, heart, and liver.
• Contains immunoglobulin domains, disulfide bonds, and is subject to glycosylation and phosphorylation, highlighting its role in signaling and adhesion.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 32–345 of the mouse Nectin-2 extracellular domain, suggesting the antibody recognizes an epitope accessible on the cell surface for flow cytometry applications.
• For flow cytometry, titrate the conjugate to determine the optimal staining concentration in your cell system.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls to set gating boundaries accurately.
• Ensure cell viability and consider Fc receptor blocking if working with FcR-expressing cells to minimize nonspecific binding.
• The antibody is specific for mouse Nectin-2; cross-reactivity with other species has not been reported.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-mouse CD112/Nectin-2 antibody supports research in immuno-oncology and cell adhesion. Explore our validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare-target raw material sourcing solutions to streamline your assay development.