ABflo 647 Anti-Mouse CD107b (LAMP2) Monoclonal Antibody for Flow Cytometry - P17047

Core Product Specifications and Parameters

Biological Background: LAMP2 (CD107b) Function and Localization

• LAMP2 (CD107b) is a highly glycosylated lysosomal membrane glycoprotein belonging to the LAMP family, also known as LGP-B, Lamp-2, and CD107b.
• It plays an essential role in lysosome biogenesis, lysosomal pH regulation, and autophagy. Related references: PMID:10972293, PMID:30718432
• Functions in chaperone-mediated autophagy by binding target proteins such as GAPDH, GPX4, NLRP3, and MLLT11 for lysosomal degradation. Related references: PMID:30718432
• Required for the fusion of autophagosomes with lysosomes during starvation-induced autophagy; deficiency leads to failure of STX17 accumulation on autophagosomes. Related references: PMID:27628032
• Necessary for normal degradation of autophagosome contents. Related references: PMID:10972293, PMID:12221139
• Localizes to lysosome, endosome, and autophagosome membranes, and is also found at the cell membrane.
• Detected predominantly in liver and kidney tissues.
• Contains disulfide bonds and multiple N-glycosylation sites; subject to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 26–189 of mouse CD107b) corresponds to the N-terminal luminal domain, which may be accessible for surface staining in flow cytometry.
• Consider using this ABflo 647 conjugate in multicolor flow cytometry panels; its far-red emission minimizes spectral overlap with common fluorophores.
• For intracellular staining, optimize permeabilization and fixation conditions to preserve epitope recognition and lysosomal membrane integrity.
• LAMP2 can translocate to the cell membrane; validation in your specific cell type and treatment conditions is recommended.
• High expression in liver and kidney suggests these as suitable positive control tissues for assay optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, offering a comprehensive range of monoclonal antibodies, recombinant proteins, and ancillary reagents to support your diagnostic development. This anti-CD107b antibody is ideal for lysosome-related research and assay development, and CamelBio can assist you with optimized antibody pairs, bulk reagents, and custom sourcing for rare targets. Whether you need validated raw materials or seamless technical support, CamelBio bridges your journey from concept to clinic.