Anti-Monoamine Oxidase B (MAOB) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P27338

Core Product Specifications and Parameters

Biological Background: Monoamine Oxidase B Function and Localization

• MAOB is a flavin-containing mitochondrial enzyme belonging to the monoamine oxidase family, requiring FAD as a cofactor for its catalytic activity.
• MAOB catalyzes the oxidative deamination of primary and some secondary amines, including neurotransmitters and the neurotoxin MPTP, with a preference for benzylamine and phenylethylamine, and generates hydrogen peroxide. Related references: PMID:11049757 PMID:11134050 PMID:20493079
• The enzyme is anchored to the mitochondrial outer membrane by a C-terminal transmembrane helix, positioning it for amine metabolism.
• Post-translational modifications such as acetylation and alternative splicing produce distinct MAOB isoforms.
• As an oxidoreductase, MAOB influences cellular redox status through the production of reactive oxygen species.
• It plays a pivotal role in catecholamine and trace amine catabolism in the central nervous system and peripheral tissues.

Experimental Guidance and Technical Tips

• The immunogen was designed from the N-terminal region (aa 1-100) of human MAOB; epitope conservation across human, mouse, and rat supports the observed cross-reactivity. Validate recognition of endogenous protein in relevant sample systems.
• For Western blotting, a specific band around ~59 kDa (UniProt predicted ~58.8 kDa) is expected; consider using tissue or cell lysates known to express MAOB, such as brain or liver.
• In immunohistochemistry (paraffin), antigen retrieval conditions may require optimization; test citrate or EDTA-based buffers at different pH levels.
• When developing ELISA assays, consider coating with the immunogen peptide or recombinant MAOB protein to calibrate antibody affinity and specificity.
• Confirm antibody performance under your experimental conditions by including appropriate positive and negative controls to account for sample-dependent variables.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated partner for IVD raw material supply, offering validated monoclonal antibodies like this anti-MAOB rabbit mAb with batch-to-batch consistency for reliable assay performance. Beyond antibodies, we provide one-stop access to antibody pairs, optimized polyclonal reagents, bulk ancillary materials, and customized sourcing of rare targets to streamline your diagnostic kit development. Leverage our expertise to advance your research in neurotransmitter metabolism, neurodegenerative disorders, or related biomarker discovery.