Anti-Monoamine Oxidase A (MAOA) Rabbit Polyclonal Antibody for IF/ICC, ELISA - P21397

Core Product Specifications and Parameters

Biological Background: Monoamine Oxidase A Function and Localization

• MAOA catalyzes the oxidative deamination of primary amines such as serotonin, playing a critical role in neurotransmitter degradation and amine metabolism in the central nervous system and peripheral tissues. Related references: PMID:18391214 PMID:20493079 PMID:24169519
• It also deaminates kynuramine to 3-(2-aminophenyl)-3-oxopropanal, which can spontaneously cyclize to 4-hydroxyquinoline.
• MAOA is a flavoprotein containing FAD as a cofactor and belongs to the monoamine oxidase family.
• The protein localizes to the mitochondrial outer membrane, with a transmembrane helix anchoring it to the membrane.
• It is expressed in tissues including heart, liver, duodenum, blood vessels, and kidney.
• Post-translational modifications include phosphorylation and acetylation, which may regulate its activity.
• Mutations in the MAOA gene are linked to Brunner syndrome, an X-linked disorder characterized by mild intellectual disability and behavioral problems.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (amino acids 1-260), which includes part of the flavin-binding domain; the antibody may recognize both native and denatured forms under appropriate conditions.
• For ELISA, consider coating with recombinant protein or cell lysates expressing MAOA; validate in the relevant sample system.
• In immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization due to mitochondrial localization; co-staining with mitochondrial markers is recommended.
• Since MAOA is a mitochondrial outer membrane protein, use mild detergents for extraction to preserve conformation.
• Alternative splicing variants exist; confirm specificity in your experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to premium IVD raw materials. This anti-MAOA polyclonal antibody exemplifies our commitment to supplying high-quality reagents for neuroscience and cardiovascular disease research. From validated antibody pairs to optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing, we cover every stage from concept to clinic.