Anti-Monoamine Oxidase A (MAOA) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P21397

Core Product Specifications and Parameters

Biological Background: Monoamine Oxidase A Function and Localization

• Monoamine oxidase A (MAO-A) is a mitochondrial outer membrane flavoprotein that catalyzes the oxidative deamination of primary and secondary amines, including key neurotransmitters like serotonin, dopamine, and norepinephrine, with concomitant reduction of O₂ to H₂O₂ (key role in neurotransmitter degradation and cellular signaling). Related references: PMID:18391214 PMID:20493079 PMID:24169519
• MAO-A preferentially oxidizes serotonin (5-hydroxytryptamine), implicating it in mood regulation and neuropsychiatric disorders.
• The enzyme also catalyzes the deamination of kynuramine to 3-(2-aminophenyl)-3-oxopropanal, which can spontaneously condense to 4-hydroxyquinoline.
• MAO-A is encoded by the MAOA gene located on the X chromosome, with alternative splicing producing isoforms; defective variants are associated with Brunner syndrome (intellectual disability and impulsive behavior).
• Subcellularly, MAO-A is anchored to the mitochondrial outer membrane via a C-terminal transmembrane helix, facing the cytoplasm.
• The protein contains a covalently bound FAD cofactor and undergoes post-translational modifications including acetylation and phosphorylation, which may regulate its activity.
• MAO-A is expressed in diverse tissues including heart, liver, duodenum, blood vessels, and kidney, reflecting its involvement in both central and peripheral amine metabolism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 428–527) of human MAOA; consider epitope accessibility in your assay format and validate binding to native and denatured protein.
• For Western blotting, the predicted molecular weight is ~60 kDa; use whole cell lysates from MAOA-expressing tissues (e.g., liver, kidney, brain) as positive controls, and confirm specificity with siRNA knockdown or knockout controls.
• In immunohistochemistry-paraffin (IHC-P), optimal antibody dilution should be empirically determined; use antigen retrieval methods and include appropriate tissue controls (e.g., human liver or brain sections).
• For ELISA, the antibody may be used as a capture or detection reagent; perform checkerboard titrations to determine optimal working concentrations and assess sandwich pair compatibility.
• Cross-reactivity with mouse and rat is reported; verify suitability for your species and application prior to large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a seamless procurement solution for IVD raw materials, including this rabbit anti-MAOA monoclonal antibody, suitable for neurotransmitter metabolism and neurobiology research. Beyond single antibodies, we supply validated antibody pairs, bulk reagents, and customizable sourcing services to accelerate your assay development from concept to clinic. Whether you need optimized monoclonal/polyclonal antibodies or rare-target raw materials, CamelBio is your trusted partner for end-to-end supply chain support.