Anti-MOGS Rabbit Polyclonal Antibody for WB and ELISA - Q13724

Core Product Specifications and Parameters

Biological Background: MOGS Function and Localization

• MOGS (mannosyl-oligosaccharide glucosidase), also known as processing A-glucosidase I, DER7, GCS1, CWH41, and CDG2B, is encoded by the MOGS gene.
• It is a glycosidase that specifically cleaves the distal alpha-1,2-linked glucose residue from the Glc3Man9GlcNAc2 oligosaccharide precursor during N-glycan degradation.
• This enzyme is localized to the endoplasmic reticulum membrane via a signal-anchor transmembrane helix, placing its catalytic domain in the ER lumen.
• MOGS belongs to the glycosyl hydrolase family and functions as a key hydrolase in glycoprotein processing.
• Mutations in MOGS are associated with congenital disorder of glycosylation type IIb (CDG2B), highlighting its critical role in glycan maturation.
• The protein undergoes alternative splicing, which may generate isoforms with potentially distinct functions or localizations.
• Its molecular weight is approximately 92 kDa (predicted MW: 91.9 kDa), consistent with its glycoprotein nature.

Experimental Guidance and Technical Tips

• For Western blotting (WB), a band around 92 kDa is expected. Consider using whole cell lysates from human, mouse, or rat cells expressing MOGS as positive controls.
• The immunogen corresponds to amino acids 60-320 of human MOGS, a region likely to be conserved across species, supporting observed cross-reactivity with mouse and rat.
• When using ELISA, consider indirect or direct coating of recombinant MOGS protein or cell lysates; validate antibody reactivity in the final assay format.
• As a polyclonal antibody, lot-to-lot variability should be assessed; inclusion of a reference standard is recommended for quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to essential IVD raw materials, including this anti-MOGS polyclonal antibody. Specializing in glycoprotein processing targets and rare disease markers, we supply validated antibodies, ancillary reagents, and custom sourcing solutions to support your assay development from early research to clinical application. Partner with us to accelerate your diagnostic projects with reliable raw materials and expert technical support.