Anti-Moesin Polyclonal Antibody for WB, IF/ICC, ELISA - P26038

Core Product Specifications and Parameters

Biological Background: Moesin Function and Localization

• Moesin, also known as membrane-organizing extension spike protein, is encoded by the MSN gene and belongs to the ezrin-radixin-moesin (ERM) protein family.
• It serves as a linker between the actin cytoskeleton and the plasma membrane, oscillating between resting and activated states to provide transient actin binding. Related references: PMID:10212266
• Phosphorylation at the C-terminal threonine triggers activation, leading to F-actin interaction and cytoskeletal reorganization that regulates cell shape, membrane transport, and signal transduction. Related references: PMID:10212266 PMID:12387735 PMID:15039356
• Moesin is crucial for immune function, influencing T and B-cell homeostasis, self-tolerance, and lymphocyte egress from lymphoid organs. It also participates in immunologic synapse formation and modulates phagolysosomal biogenesis in macrophages. Related references: PMID:9298994 PMID:9616160 PMID:27405666
• Subcellularly, moesin localizes to the cell membrane, cytoplasm, cytoskeleton, apical cell membrane, and microvilli, consistent with its role in organizing membrane-associated actin structures.
• Moesin is subject to post-translational modifications including phosphorylation, acetylation, and S-nitrosylation, which may regulate its activity and interactions.
• The protein is ubiquitously expressed across all human tissues and cultured cells, reflecting its fundamental role in cellular architecture.
• Moesin is linked to disease variants and host-virus interactions, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 458–557) of human moesin, encompassing the critical threonine phosphorylation site. The antibody may detect both phosphorylated and unphosphorylated forms; validate under relevant conditions.
• For Western blot, the predicted molecular weight is approximately 68 kDa. Use appropriate positive and negative controls and optimize loading amounts for the species of interest.
• In immunofluorescence/ICC, ensure proper fixation and permeabilization protocols to preserve epitope accessibility. Counterstain with actin or membrane markers to confirm localization.
• For ELISA, consider the polyclonal nature for increased sensitivity; however, pretest with recombinant protein or cell lysates to confirm reactivity in the desired assay format.
• Cross-reactivity with mouse and rat has been reported, but validation in the specific experimental system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, connecting diagnostic manufacturers and research institutions with high-quality antibodies and reagents. For targets like moesin, a key regulator of cytoskeletal dynamics and immune function, we supply validated anti-moesin polyclonal antibodies as well as matched monoclonal pairs, along with bulk ancillary reagents and specialized sourcing for rare targets, streamlining your development from concept to clinic.