Anti-Moesin Polyclonal Antibody for WB, ELISA - P26038

Core Product Specifications and Parameters

Biological Background: Moesin Function and Localization

• Moesin (MSN) is a member of the ezrin-radixin-moesin (ERM) family that tethers actin filaments to the plasma membrane, oscillating between resting and activated states to provide transient cytoskeletal interactions. Related references: PMID:10212266
• Phosphorylation of moesin on its C‑terminal threonine induces activation, leading to F‑actin binding and subsequent cytoskeletal rearrangement. Related references: PMID:10212266
• These rearrangements control cell shape determination, membrane transport, and signal transduction. Related references: PMID:12387735, PMID:15039356
• Moesin plays a critical role in immunity by regulating T‑ and B‑cell homeostasis, self‑tolerance, and lymphocyte egress from lymphoid organs. Related references: PMID:9298994, PMID:9616160
• The protein contributes to immunologic synapse formation, facilitating effective immune cell communication. Related references: PMID:27405666
• Subcellularly, moesin localizes to the cell membrane, apical cell membrane, cytoplasm, and cytoskeleton, and is enriched in microvilli. It is expressed in all tissues and carries multiple post‑translational modifications including phosphorylation, acetylation, and S‑nitrosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal domain (aa 1–300); consider this when interpreting band patterns, as the antibody may detect both full‑length and N‑terminal fragments.
• For Western blotting, validate the antibody in lysates from human or mouse cell lines, given the reported cross‑reactivity. The expected molecular weight is ~68 kDa, but verify with appropriate controls.
• For ELISA, optimize the coating antigen and antibody dilution using recombinant moesin protein. Perform titration experiments to achieve the desired sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For moesin‑related research—spanning cytoskeleton dynamics, immune regulation, and cell signaling—we offer targeted sourcing support to streamline your assay development from concept to clinic.