Anti-MOB4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y3A3

Core Product Specifications and Parameters

Biological Background: MOB4 (MOB-like protein phocein) Function and Localization

• MOB4, also known as phocein, preimplantation protein 3, or MOBKL3, is a member of the MOB family of kinase activator-like proteins.
• It is a core component of the striatin-interacting phosphatase and kinase (STRIPAK) complexes, which regulate protein phosphorylation and dephosphorylation events.
• Through STRIPAK, MOB4 participates in modulating multiple signaling cascades, including the Hippo, MAPK, and nuclear receptor pathways, as well as cytoskeleton remodeling.
• STRIPAK complexes containing MOB4 are implicated in diverse biological processes such as cell growth, differentiation, apoptosis, metabolism, and immune regulation.
• MOB4 localizes predominantly to the cytoplasm and perinuclear region, and is also found associated with the Golgi apparatus and Golgi stack membranes.
• The protein is subject to phosphorylation and binds metal ions such as zinc, features consistent with its regulatory roles.
• Alternative splicing of the MOB4 gene generates multiple transcript variants, indicating potential isoform diversity.

Experimental Guidance and Technical Tips

• For Western blot, the expected band is approximately 26 kDa. Validate with appropriate positive controls, as expression levels may vary.
• In immunohistochemistry (paraffin-embedded sections), optimize antigen retrieval and consider the perinuclear/Golgi staining pattern.
• For ELISA, pair with a matched detection antibody or antigen, and titrate antibody concentration.
• The antibody is raised against a human sequence but cross-reacts with mouse and rat; verify cross-reactivity in your sample system.
• As a polyclonal antibody, lot-to-lot variability may occur; validate each new lot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for diagnostic development. For researchers studying MOB4 and STRIPAK signaling, we facilitate access to rare target raw materials and technical consultation to accelerate your assay development pipeline.