Anti-MOB1A/B Monoclonal Antibody for WB, ELISA - Q9H8S9 / Q7l9L4

Core Product Specifications and Parameters

Biological Background: MOB1A/B Function and Localization

• MOB1A (gene: MOB1A) and MOB1B (gene: MOB1B) are conserved adaptor proteins that serve as core components of the Hippo tumor suppressor pathway, which controls organ size, cell proliferation, and apoptosis.
• MOB1A/B bind and activate the LATS1/2 kinases, leading to phosphorylation and cytoplasmic sequestration of the YAP1 and WWTR1/TAZ transcriptional co-activators, thereby inhibiting pro-growth gene expression.
• In addition to its role in Hippo signaling, MOB1A stimulates the kinase activity of STK38 and STK38L, and cooperates with STK3/MST2 to activate STK38, indicating broader regulatory functions in cell signaling.
• MOB1A is broadly expressed in adult and fetal tissues, including adrenal gland, brain, thymus, testis, heart, and placenta, with specific isoforms detected in fetal brain and liver.
• Post-translational modifications of MOB1A encompass phosphorylation, acetylation, and zinc metal binding, which may modulate its activity and interactions within signaling complexes.
• Alternative splicing of MOB1A produces multiple isoforms, potentially contributing to functional diversity across different cell types and developmental stages.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the N-terminal region (aa 1-100) of human MOB1A/B, likely conferring reactivity against both MOB1A and MOB1B, as well as cross-reactivity with mouse and rat orthologs.
• For Western blot analysis, consider using whole-cell lysates from cell lines or tissues known to express MOB1A/B, such as thymus or testis; a band at approximately 25 kDa is expected.
• When performing ELISA, validate the antibody's binding specificity by testing against recombinant MOB1A/B protein or using a competitive peptide blocking assay.
• This antibody may be suitable for detecting endogenously expressed MOB1A/B under various experimental conditions, but the optimal dilution and incubation parameters should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive raw materials partner for IVD manufacturers, offering validated antibody pairs, bulk ancillary reagents, and custom sourcing of rare targets. This MOB1A/B monoclonal antibody supports research into Hippo signaling, cell cycle regulation, and cancer biology. CamelBio's integrated supply chain ensures reliable access to high-quality antibodies, recombinant proteins, and technical consulting to streamline your diagnostic development from concept to clinic.