Anti-MNX1/HB9/HLXB9 Polyclonal Antibody for WB and ELISA - P50219

Core Product Specifications and Parameters

Biological Background: MNX1/HB9/HLXB9 Function and Localization

• Motor neuron and pancreas homeobox protein 1 (MNX1), also known as Homeobox protein HB9 (HLXB9), is a transcription factor belonging to the homeobox family.
• Key roles in establishing motor neuron identity, acting in concert with LIM domain transcription factor LMO4, and negatively modulating transcription of interneuron genes in motor neurons by blocking regulatory sequence interactions of the ISL1-LHX3 complex.
• Involved in pancreas development and function, potentially influencing pancreatic cell fate specification.
• Localized primarily in the nucleus, consistent with its function as a DNA-binding transcription factor.
• Expressed in lymphoid and pancreatic tissues, with additional neural expression implied by its motor neuron functions.
• Subject to post-translational modifications including acetylation and phosphorylation, which may regulate its activity; alternative splicing and disease-associated variants have been reported.

Experimental Guidance and Technical Tips

• For western blot (WB) applications, consider using whole-cell lysates from motor neuron-related cell lines (e.g., NSC-34) or pancreatic cell lines (e.g., PANC-1) to detect endogenous MNX1.
• Validate antibody specificity in the relevant species (human, mouse, rat) by comparing signals in positive versus negative control tissues or cell lines.
• For ELISA, optimize coating antigen and antibody dilutions using recombinant MNX1 protein or peptide immunogen as a standard.
• The immunogen (aa 201-315) lies within a region that may be conserved across species; cross-reactivity with mouse and rat is expected, but confirm with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For research involving motor neuron development or pancreatic function, we supply high-quality antibodies like this anti-MNX1/HB9/HLXB9 polyclonal antibody, along with validated antibody pairs, optimized monoclonal/polyclonal options, bulk ancillary reagents, and sourcing of rare-target raw materials. Let CamelBio be your strategic partner in accelerating diagnostic development and translational research.