Anti-MMP9 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P14780

Core Product Specifications and Parameters

Biological Background: MMP9 Function and Localization

• Matrix metalloproteinase-9 (MMP9), also known as gelatinase B (GELB) or 92 kDa type IV collagenase, belongs to the matrix metalloproteinase family.
• It plays a critical role in the degradation of extracellular matrix components, facilitating leukocyte migration and tissue remodeling. Related references: PMID:12879005 PMID:1480034 PMID:2551898
• MMP9 specifically cleaves type IV and type V collagen, generating characteristic fragments, and degrades fibronectin. Related references: PMID:1480034
• It processes KiSS1 and NINJ1, releasing bioactive peptides involved in cell motility and inflammation. Related references: PMID:12879005 PMID:32883094
• MMP9 is a secreted enzyme found in the extracellular space and matrix, produced primarily by neutrophils, alveolar macrophages, and granulocytes. Related references: PMID:7683678
• As a zinc-dependent metalloprotease, MMP9 requires calcium for stability and is synthesized as an inactive zymogen that undergoes proteolytic activation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 538–707 of human MMP9, located in the C-terminal hemopexin-like domain. This region may detect both latent and active forms; however, validation in your specific experimental system is advised.
• For Western blotting, a band near 78 kDa is expected for the full-length pro-enzyme, but processed forms may appear at lower molecular weights. Include appropriate positive controls (e.g., neutrophil or macrophage lysates).
• For immunohistochemistry and immunofluorescence, consider antigen retrieval protocols and validate specificity using MMP9-knockout tissues or blocking peptides if available.
• This antibody cross-reacts with mouse and rat; optimize dilutions for each species and confirm reactivity in relevant disease models where MMP9 is upregulated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this MMP9 rabbit polyclonal antibody as part of a comprehensive portfolio of IVD raw materials. For diagnostic developers and researchers, we provide bulk antibody supply, custom conjugations, and ancillary reagents to support assay development in areas such as extracellular matrix biology and inflammatory diseases.