Anti-MMP7 (Matrilysin) Rabbit Monoclonal Antibody for WB, ELISA - P09237

Core Product Specifications and Parameters

Biological Background: MMP7 Function and Localization

• Matrilysin (MMP-7), encoded by the MMP7 gene, is a secreted matrix metalloproteinase involved in extracellular matrix remodeling. It degrades gelatins of types I, III, IV, and V, fibronectin, and casein.
• MMP-7 can activate procollagenase, further promoting collagen degradation in tissue environments.
• It is synthesized as an inactive zymogen and requires proteolytic activation to achieve enzymatic function.
• Subcellularly, MMP-7 is secreted into the extracellular space and localizes to the extracellular matrix.
• The protein binds calcium and zinc ions, which are essential for its metalloprotease activity, as noted in UniProt keywords.
• According to UniProt, the mature protein has a calculated molecular weight of ~29.7 kDa, consistent with observed ~30 kDa in western blotting.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to full‑length human MMP7 (residues 1–267), suggesting that the antibody likely recognizes the native or denatured full‑length protein.
• For western blotting, consider using reducing conditions and appropriate sample preparation—MMP7 is a secreted protein; thus, cell culture supernatant or serum/plasma samples may be suitable.
• As a monoclonal antibody, it is expected to provide consistent specificity; validate the antibody’s performance in your specific sample system and application, as performance may vary.
• Although not listed as validated, the antibody might be suitable for other applications such as immunohistochemistry or immunoprecipitation if cross‑reactivity and specificity are confirmed experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and research institutions with high‑quality IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents. This MMP7 monoclonal antibody is part of our extensive portfolio for matrix biology and cancer research applications. Through our one‑stop sourcing service, we help streamline your procurement of rare‑target and routine raw materials, supporting your development from concept to clinic.