Anti-MMP3 (Stromelysin-1) Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P08254

Core Product Specifications and Parameters

Biological Background: Stromelysin-1 Function and Localization

• Stromelysin-1 (MMP-3) is a secreted zinc-dependent endopeptidase belonging to the matrix metalloproteinase (MMP) family, also known as transin-1.
• It degrades a broad spectrum of extracellular matrix (ECM) components, including fibronectin, laminin, gelatins (types I, III, IV, V), collagens (III, IV, X, IX), and cartilage proteoglycans; it also activates other molecules such as pro-MMP9 and growth factors. Related references: PMID:11029580, PMID:1371271
• The inactive proenzyme is activated in the extracellular space by the plasmin cascade signaling pathway. Related references: PMID:2383557
• MMP3 also functions intracellularly: in dopaminergic neurons, stress-induced activation by HTRA2 serine protease contributes to neurodegeneration and α-synuclein cleavage. Related references: PMID:22265821, PMID:21330369
• It plays a role in innate immunity and possesses antiviral activity against viruses such as vesicular stomatitis virus, influenza A (H1N1), and herpes simplex virus 1; mechanistically, it translocates from the cytoplasm into the nucleus to modulate NF-κB signaling. Related references: PMID:35940311
• Subcellularly, MMP3 is localized to the extracellular matrix, nucleus, and cytoplasm.
• Key structural and functional features include calcium and zinc binding, a disulfide bond, and a cysteine switch that maintains the zymogen form.

Experimental Guidance and Technical Tips

• For Western blot (WB) analysis, use reducing conditions and validate with MMP3-expressing cell lines or tissue lysates (e.g., fibroblasts, chondrocytes) from human, mouse, or rat.
• In immunohistochemistry (IHC-P), antigen retrieval (heat- or enzyme-mediated) is often required; titrate the antibody and include appropriate positive and negative tissue controls.
• For immunofluorescence (IF/ICC), fix and permeabilize cells under optimized conditions; consider secretion inhibitors if targeting the intracellular pool of MMP3.
• This monoclonal antibody, raised against the C-terminal region (aa 385–477), can be employed in ELISA formats; assess its performance as a capture or detection reagent in sandwich ELISA setups.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, delivering this high-quality MMP3 monoclonal antibody together with complementary reagents such as recombinant proteins and ELISA kits. Our services extend to custom antibody development and sourcing of rare targets, facilitating research in ECM degradation, immune response, and neurodegeneration.