Anti-MMP3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P08254

Core Product Specifications and Parameters

Biological Background: MMP3 Function and Localization

• Stromelysin-1 (MMP3) is a secreted matrix metalloproteinase with broad substrate specificity, capable of degrading fibronectin, laminin, gelatins of type I, III, IV, and V, collagens III, IV, X, and IX, and cartilage proteoglycans. It also activates proMMP9 and growth factors. Related references: PMID:11029580, PMID:1371271
• Synthesized as an inactive zymogen, MMP3 is activated extracellularly through the plasmin cascade signaling pathway, enabling its proteolytic functions in tissue remodeling. Related references: PMID:2383557
• Beyond extracellular roles, MMP3 acts intracellularly; in dopaminergic neurons, stress-activated HTRA2 protease processes MMP3, leading to microglial activation and alpha-synuclein cleavage, contributing to neurodegeneration. Related references: PMID:22265821, PMID:21330369
• MMP3 participates in innate immunity by exerting antiviral activity against vesicular stomatitis virus, influenza A virus (H1N1), and human herpes virus 1. It translocates from the cytoplasm to the nucleus upon infection to modulate NF-kappa-B signaling. Related references: PMID:35940311
• Subcellularly, MMP3 is found in the extracellular matrix, secreted, and also localizes to the nucleus and cytoplasm, reflecting its diverse functional compartments.
• Structural features include calcium and zinc binding sites, disulfide bonds, and a signal peptide for secretion, classifying it as a member of the metzincin superfamily.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 385-477) of human MMP3, suggesting potential recognition of both full-length and cleaved forms; consider validation in relevant sample systems.
• For Western blotting, note that MMP3 has a predicted molecular weight of ~54 kDa, but may appear as multiple bands due to activation and glycosylation; optimize loading controls and antibody dilution.
• In immunohistochemistry and immunofluorescence, pre-test antigen retrieval conditions and include appropriate controls to distinguish secreted and intracellular MMP3 pools.
• For ELISA, pair with a suitable capture antibody; validate sensitivity and specificity using recombinant protein or known positive samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials and technical support—from concept to clinic. This MMP3 Rabbit pAb targets a key protease involved in extracellular matrix remodeling and immunity, supporting research in cancer, arthritis, and infectious diseases. In addition to this polyclonal antibody, we offer validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your IVD development and production needs.