Anti-MMP25 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NPA2

Core Product Specifications and Parameters

Biological Background: MMP25 Function and Localization

• MMP25, also designated MT6-MMP or leukolysin, is a membrane-type matrix metalloproteinase belonging to the MMP family.
• It is anchored to the cell membrane via a carboxyl-terminal GPI moiety and can also be shed into the extracellular space and matrix.
• Its catalytic activity is dependent on zinc and calcium ions, consistent with metalloprotease classification.
• The primary reported substrate is progelatinase A, suggesting a role in extracellular matrix remodeling.
• MMP25 is predominantly expressed in leukocytes, lung, and spleen, with additional detection in colon carcinoma, astrocytoma, and glioblastoma.
• Post-translational modifications include glycosylation, disulfide bond formation, and cleavage on a pair of basic residues for zymogen activation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 350–540 within the hemopexin-like domain; for western blot, anticipate a band around 63 kDa under reducing conditions.
• In immunofluorescence/ICC, expect a cell surface and possibly punctate intracellular pattern; co-stain with organelle markers to confirm localization.
• Since expression is prominent in leukocytes and certain tumors, consider validating in PBMC lysates or glioblastoma/colon carcinoma cell lines.
• For ELISA, use recombinant protein or cell lysates from positive controls; assay conditions should be optimized empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers and research institutions with a seamless supply chain for IVD raw materials. This anti-MMP25 antibody supports research into oncology and immune cell biology, and CamelBio can source additional validated antibody pairs, bulk ancillary reagents, or custom rare-target materials tailored to your project needs. Let us help streamline your workflow from concept to clinical application.