Anti-MMP20 Rabbit Polyclonal Antibody for WB, ELISA - O60882

Core Product Specifications and Parameters

Biological Background: MMP20 Function and Localization

• Matrix metalloproteinase-20 (MMP20), also known as enamelysin or enamel metalloproteinase, is a secreted protease that belongs to the matrix metalloproteinase family.
• It plays a central role in tooth enamel formation by degrading amelogenin, the major protein component of the enamel matrix, and also cleaves cartilage extracellular matrix proteins such as aggrecan and cartilage oligomeric matrix protein (COMP).
• The enzyme is expressed specifically in the enamel organ, highlighting its critical function in dental tissue development and mineralization.
• Subcellularly, MMP20 is secreted into the extracellular space and extracellular matrix, where it exerts its proteolytic activity.
• Structurally, MMP20 shares typical MMP features including a signal peptide, propeptide, catalytic domain with zinc-binding motif, hemopexin-like repeats, and requires calcium and zinc for activity.
• Post-translational modifications include autocatalytic activation from zymogen form and disulfide bond formation, and mutations in the MMP20 gene are associated with amelogenesis imperfecta, a disorder of enamel formation.
• Keywords related to MMP20 include metalloprotease, hydrolase, extracellular matrix, secreted, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 108–310 of human MMP20; consider this when evaluating epitope accessibility in native versus denatured samples.
• For Western blot (WB), validate detection of the ~54 kDa band in relevant enamel or dental tissue lysates, and use positive controls expressing MMP20.
• In ELISA applications, assess binding to recombinant MMP20 or native protein in biological fluids, and optimize coating and blocking conditions.
• Cross-reactivity is reported for human, mouse, and rat; confirm reactivity in your specific experimental model, as sequence homology may affect affinity.
• Due to MMP20’s role in enamel formation, consider using dental germ or odontoblast cell lines for functional studies.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio connects diagnostic manufacturers and researchers with high-quality antibodies like this MMP20 polyclonal antibody for dental and skeletal disease research. We offer one-stop access to validated antibody pairs, bulk ancillary reagents, and sourcing of rare target materials, supporting your projects from concept to clinic. Contact us to streamline your procurement and accelerate assay development with reliable immunoreagents.