Anti-MMP2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P08253

Core Product Specifications and Parameters

Biological Background: MMP2 Function and Localization

• MMP2, also known as 72 kDa type IV collagenase or gelatinase A, is a member of the matrix metalloproteinase (MMP) family. It is encoded by the MMP2 gene, which undergoes alternative splicing.
• It degrades extracellular matrix components such as type IV collagen and gelatin, and also acts on nonmatrix proteins including big endothelin-1 and beta-type CGRP, promoting vasoconstriction.
• MMP2 plays critical roles in tissue remodeling, angiogenesis, tumor invasion, inflammation, and atherosclerotic plaque rupture.
• The C-terminal non-catalytic fragment (PEX) possesses anti-angiogenic and anti-tumor properties, inhibits cell migration, and serves as a ligand for integrin αvβ3 on blood vessels.
• MMP2 mediates proteolysis of CHUK/IKKA, initiating innate immune signaling via NF-kappaB, NFAT, and IRF pathways in response to mitochondrial-nuclear stress.
• Subcellular localization includes secretion into the extracellular space, association with the extracellular matrix and membrane, and presence in the nucleus, cytoplasm, and mitochondria.
• Post-translational modifications include phosphorylation, glycosylation, autocatalytic cleavage, and disulfide bond formation; the protein contains calcium and zinc binding sites.

Experimental Guidance and Technical Tips

• For Western blot, consider that the antibody may recognize both full-length MMP2 (74 kDa) and cleaved fragments (e.g., PEX) due to its C-terminal immunogen. Validate in the relevant sample system.
• For IF/ICC, MMP2 is secreted but also associates with cell membranes and matrix; optimize fixation and permeabilization conditions to capture these localizations.
• For ELISA, MMP2 is present in extracellular fluids; this antibody could be tested as part of a sandwich ELISA with an appropriate capture antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing high-quality antibodies and reagents for diagnostic development. In addition to this MMP2 antibody, we offer validated antibody pairs, monoclonal options, and bulk ancillary reagents to support your MMP2-related research and assay development. Rely on CamelBio to streamline your supply chain from concept to clinic.