Anti-MMP2 Rabbit Monoclonal Antibody for WB, ELISA - P08253

Core Product Specifications and Parameters

Biological Background: MMP2 Function and Localization

• Protein identity and isoforms: MMP2 encodes the 72 kDa type IV collagenase, also known as gelatinase A, MMP-2, or TBE-1. It is a ubiquitously expressed metalloproteinase belonging to the matrix metalloproteinase family.
• Core enzymatic functions: MMP-2 degrades extracellular matrix components such as type IV collagen and gelatin, and plays key roles in tissue remodeling, angiogenesis, tumor invasion, wound healing, and atherosclerotic plaque rupture. It also cleaves non-matrix substrates like big endothelin-1, beta-type CGRP, CHUK/IKKA, and GSK3beta, influencing vasoconstriction, innate immunity, and myocardial oxidative stress.
• Subcellular localization: MMP-2 is secreted into the extracellular space and matrix but is also found at the membrane, nucleus, cytoplasm, and mitochondria, reflecting its multifaceted roles.
• Tissue specificity and pathological expression: Produced by normal skin fibroblasts; the C-terminal PEX fragment is expressed in various tumors, including gliomas, breast, and prostate cancers.
• Anti-angiogenic PEX domain: The non-catalytic PEX fragment of MMP-2 acts as a ligand for integrin αv/β3, inhibiting angiogenesis, tumor growth, cell migration, and adhesion to FGF2 and vitronectin.
• Post-translational modifications and structural features: MMP-2 is a calcium-dependent zinc-endopeptidase synthesized as a zymogen. It undergoes autocatalytic cleavage for activation and contains disulfide bonds, glycosylation sites, and phosphorylation modifications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 500–600 within the C-terminal hemopexin-like domain (PEX) of human MMP-2. This region is likely to detect both the full-length pro-form (~74 kDa) and active forms after cleavage of the pro-domain.
• For Western blot, consider that MMP-2 may appear as a doublet or multiple bands due to glycosylation and proteolytic processing; validate the detected bands against appropriate controls in your specific sample system.
• Given the monoclonal nature of this antibody, it offers consistent batch-to-batch reproducibility. Confirm optimal working dilutions by titration in your assay conditions.
• Cross-reactivity with mouse and rat samples is supported, reflecting sequence conservation in the epitope region. However, reactivity should be verified experimentally for each species and tissue type.
• For ELISA applications, this antibody can be used as a capture or detection reagent; pairing with another clone or polyclonal antibody is recommended for sandwich assays. Validate performance with recombinant MMP-2 or native samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents to support diagnostic manufacturers and research laboratories. This anti-MMP2 antibody, targeting a key metalloproteinase in cancer, cardiovascular, and inflammation research, exemplifies our commitment to supplying high-quality reagents for demanding applications. Whether you need rare target raw materials or comprehensive technical consultation, CamelBio bridges the gap from concept to clinic.