Anti-MMP2 Rabbit Monoclonal Antibody for WB and ELISA - P08253

Core Product Specifications and Parameters

Biological Background: MMP2 Function and Localization

• MMP2, also known as 72 kDa type IV collagenase, gelatinase A, and CLG4A, is a ubiquitously expressed matrix metalloproteinase that plays critical roles in extracellular matrix degradation and tissue remodeling.
• Core functions include remodeling of the vasculature, promotion of angiogenesis, facilitation of tissue repair, tumor invasion and metastasis, inflammation, and atherosclerotic plaque rupture. It also processes non-matrix substrates such as big endothelin-1 and β-type CGRP, promoting vasoconstriction, and cleaves KISS at a Gly-Leu bond.
• The C-terminal PEX domain exerts anti-angiogenic and anti-tumor activities by inhibiting cell migration and adhesion to FGF2 and vitronectin, acting as a ligand for integrin αvβ3 on blood vessels.
• MMP2 mediates proteolysis of CHUK/IKKA, triggering a primary innate immune response via mitochondrial-nuclear stress signaling and activation of NF-κB, NFAT, and IRF transcriptional pathways.
• Subcellular localization includes secretion into the extracellular space/ matrix, as well as presence in membrane, nucleus, cytoplasm, and mitochondria.
• MMP2 is produced by normal skin fibroblasts, and its PEX fragment is expressed in various tumors including gliomas, breast, and prostate cancers.
• Key structural features include a signal peptide, propeptide (zymogen form), catalytic domain with zinc-binding site, fibronectin type-II domain, and a hemopexin-like C-terminal domain; it undergoes autocatalytic cleavage and multiple phosphorylation modifications.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal hemopexin-like domain (aa 451–660), which is unique to MMP2 among MMP family members; this region is expected to confer target specificity in western blotting and ELISA applications.
• For western blotting of MMP2, consider including appropriate positive controls (e.g., lysates from human, mouse, or rat tissues/cells known to express MMP2, such as fibroblasts or tumor lines) and use reducing conditions to detect the ~74 kDa pro-form and ~64 kDa active form.
• In ELISA, pair this antibody with a suitable detection antibody (e.g., another clone recognizing a distinct epitope) for sandwich formats; pre-validation of the antibody pair in the relevant sample matrix is recommended.
• Since MMP2 is secreted, consider preparing conditioned media or serum-starved culture supernatants to enrich for the protein, particularly when probing for endogenous levels in low-abundance samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research teams with validated IVD raw materials essential for MMP2-related research in cancer, cardiovascular disease, and tissue remodeling. Our portfolio includes optimized monoclonal antibodies, matched antibody pairs, and bulk ancillary reagents, all accessible through a streamlined sourcing channel. For custom or rare targets, our technical team facilitates procurement from concept to clinic, ensuring reliable supply continuity.