Anti-MMP16 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P51512

Core Product Specifications and Parameters

Biological Background: Matrix Metalloproteinase-16 Function and Localization

• MMP16, also known as MT3-MMP or MMP-X2, is a member of the matrix metalloproteinase family.
• It encodes a membrane-type matrix metalloproteinase with a transmembrane domain, anchoring it to the cell surface, and a secreted isoform.
• Functions as an endopeptidase that degrades extracellular matrix components, notably collagen type III and fibronectin.
• Activates progelatinase A, contributing to matrix remodeling in blood vessels.
• The short isoform cleaves fibronectin and collagen type III at a lower rate, and upon interaction with CSPG4, may facilitate degradation of type I collagen by melanoma cells.
• Subcellularly, MMP16 localizes to the cell membrane, cell surface, and is also secreted into the extracellular space and matrix.
• Tissue expression is detected in heart, brain, placenta, ovary, and small intestine; the short isoform is specifically found in the ovary.
• Key structural features include calcium and zinc binding, disulfide bonds, glycosylation, and cleavage on basic residues for activation from zymogen.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 290–565 of human MMP16, a region likely encompassing the catalytic and hemopexin-like domains. Consider this when designing complementary detection reagents.
• For Western blotting, expect a band near 70 kDa; use tissue lysates from heart, brain, or placenta as positive controls.
• In immunohistochemistry and immunocytochemistry, optimize antigen retrieval and permeabilization conditions to expose the membrane-bound or secreted epitope.
• As a polyclonal antibody, it may offer high sensitivity but exhibit lot-to-lot variability; validate performance in the relevant sample system.
• For ELISA applications, verify compatibility with your sample matrix and include proper controls for matrix metalloproteinase activity if needed.
• Cross-reactivity with mouse is reported; confirm specificity when working with mouse tissues or cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, labs, and research institutes to provide reliable, one-stop access to IVD raw materials. This MMP16 antibody supports research into extracellular matrix remodeling, cancer invasion, and vascular biology. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare target raw materials—all designed to streamline your development from concept to clinic.