Anti-MMP14/MT1-MMP Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P50281

Core Product Specifications and Parameters

Biological Background: MMP14/MT1-MMP Function and Localization

• Matrix metalloproteinase-14 (MMP14), also designated MT1-MMP, is a membrane-type metalloproteinase synthesized as a zymogen that requires proteolytic cleavage for activation. It contains characteristic disulfide bonds, calcium‑ and zinc‑binding sites, and is regulated by phosphorylation.
• As an endopeptidase, MMP14 degrades various extracellular matrix components, most notably collagen, and is essential for pericellular collagenolysis and connective tissue remodeling (PubMed:8015608).
  Related references:
  PMID:8015608
• MMP14 activates pro‑gelatinase A (pro‑MMP2) by proteolytic cleavage, thereby functioning as a positive regulator of cell growth and migration. It also contributes to the formation of fibrovascular tissues together with pro‑MMP2 (PubMed:22065321, PubMed:12714657).
  Related references:
  PMID:22065321
  PMID:12714657
• The enzyme acts as a regulator of Notch signaling by mediating cleavage and inhibition of the ligand DLL1 (PubMed:21572390).
  Related references:
  PMID:21572390
• MMP14 cleaves ADGRB1 to release vasculostatin‑40, an angiogenesis inhibitor, and also cleaves and inactivates the GDF15 receptor GFRAL, modulating the GDF15‑GFRAL aversive response (PubMed:22330140, PubMed:35177851).
  Related references:
  PMID:22330140
  PMID:35177851
• Localization is primarily at the cell membrane, but MMP14 is also detected in melanosomes and the cytoplasm, consistent with its role in pericellular proteolysis and intracellular signaling.
• Expression is prominent in stromal cells of the colon, breast, and head and neck, as well as in lung tumors, aligning with its function in tumor microenvironment remodeling.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to amino acids 75–174 within the extracellular domain of human MMP14, a region conserved across human, mouse, and rat – consistent with the reported cross‑reactivity.
• For western blotting, the pro‑enzyme runs around 66 kDa; active, processed forms may appear at lower molecular weights. Validate band patterns using relevant cell or tissue lysates (e.g., HT‑1080 fibrosarcoma cells, placental tissue).
• In immunohistochemistry (IHC‑P), antigen retrieval may be necessary; test on paraffin‑embedded sections of colon, breast, or lung tumor samples. Stromal staining is expected.
• For immunofluorescence (IF/ICC), permeabilization is required to detect cytoplasmic and membranous MMP14. Use appropriate isotype controls.
• ELISA applications should consider pairing with a capture antibody against a distinct epitope; the antibody’s polyclonal nature may offer robust signal when used as a detector.
• As with all polyclonal antibodies, lot‑to‑lot consistency should be verified in the intended assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined, one‑stop avenue for sourcing high‑quality IVD raw materials. This anti‑MMP14/MT1‑MMP polyclonal antibody supports studies into cancer invasion, angiogenesis, and tissue remodeling. Beyond individual reagents, we offer validated antibody pairs, bulk ancillary materials, and custom sourcing for rare targets, helping you accelerate assay development from concept to clinic.