Anti-MMP14/MT1-MMP Monoclonal Antibody for WB, IHC-P, FC, ELISA - P50281

Core Product Specifications and Parameters

Biological Background: MMP14/MT1-MMP Function and Localization

• Membrane-type matrix metalloproteinase, also known as MMP-X1, MT-MMP 1, MT1-MMP, and MT1MMP.
• Endopeptidase that degrades various components of the extracellular matrix such as collagen; essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development. Related references: PMID:8015608
• Activates progelatinase A/MMP2, acting as a positive regulator of cell growth and migration, and is involved in the formation of fibrovascular tissues in association with pro-MMP2. Related references: PMID:22065321, PMID:8015608, PMID:12714657
• May be involved in actin cytoskeleton reorganization by cleaving PTK7. Related references: PMID:20837484
• Acts as a regulator of Notch signaling by mediating cleavage and inhibition of DLL1. Related references: PMID:21572390
• Cleaves ADGRB1 to release vasculostatin-40, which inhibits angiogenesis. Related references: PMID:22330140
• Acts as a negative regulator of the GDF15-GFRAL aversive response by mediating cleavage and inactivation of GFRAL. Related references: PMID:35177851
• Localizes to the cell membrane, melanosomes, and cytoplasm. Expressed in stromal cells of colon, breast, and head and neck; also found in lung tumors.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 100-200 of human MMP14, which may detect both pro and active forms.
• For Western blot, the predicted molecular weight is ~66 kDa; consider using reducing conditions and appropriate positive controls (e.g., human, mouse, or rat tissue lysates).
• For immunohistochemistry (IHC-P), validate in tissue sections from colon, breast, or lung tumors, where MMP14 is known to be expressed.
• For flow cytometry, note the cell surface localization; fixation and permeabilization protocols may influence detection sensitivity.
• ELISA applications may be suitable for detecting MMP14 in biological samples; consider sandwich pair optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop access to IVD raw materials, including this MMP14/MT1-MMP rabbit monoclonal antibody for extracellular matrix and cancer invasion research. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist with rare target sourcing to support your diagnostic development from concept to clinic.