Anti-MMP14 Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - P50281

Core Product Specifications and Parameters

Biological Background: MMP14 Function and Localization

• MMP14 (Matrix metalloproteinase-14) is also known as MT1-MMP, MT-MMP 1, and MMP-X1, a transmembrane zinc-dependent endopeptidase.
• It functions as a key pericellular collagenase, degrading various extracellular matrix components such as collagen, which is essential for connective tissue remodeling during development. Related references: PMID:8015608
• MMP14 activates pro-MMP2 (gelatinase A) by proteolytic cleavage, thereby promoting cell migration and invasion. Related references: PMID:22065321, PMID:8015608, PMID:12714657
• It modulates Notch signaling by cleaving and inhibiting DLL1, and negatively regulates the GDF15-GFRAL aversive response via GFRAL cleavage. Related references: PMID:21572390, PMID:35177851
• MMP14 is involved in actin cytoskeleton reorganization by cleaving PTK7 and releases the anti-angiogenic vasculostatin-40 from ADGRB1. Related references: PMID:20837484, PMID:22330140
• It localizes to the cell membrane, melanosomes, and cytoplasm, and is expressed in stromal cells of colon, breast, head and neck, as well as lung tumors.
• MMP14 contains a signal peptide, a prodomain cleaved during zymogen activation, a catalytic domain with a zinc-binding motif, and a hemopexin-like repeat domain; it undergoes phosphorylation and disulfide bond formation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 21-538, covering the entire extracellular domain including the catalytic and hemopexin domains, which may yield antibodies recognizing multiple epitopes and potentially the pro-form and active enzyme.
• For Western blotting, consider using reducing and denaturing conditions to resolve the predicted 66kDa protein; note that glycosylation or proteolytic processing may produce additional bands.
• In immunofluorescence (IF/ICC) and IF-P, membrane staining is expected; permeabilization may be required for cytoplasmic detection.
• ELISA can be designed with this polyclonal antibody as capture or detection reagent, but appropriate validation in the sample matrix is essential.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive, one-stop platform for IVD raw material sourcing and technical support—streamlining the path from concept to clinic. This anti-MMP14 antibody is an example of our broad portfolio supporting ECM remodeling and oncology research, and we can also supply validated antibody pairs, optimized monoclonal/polyclonal preparations, bulk ancillary reagents, and custom sourcing for rare targets.