Anti-MMP13 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P45452

Core Product Specifications and Parameters

Biological Background: MMP13 Function and Localization

• Collagenase 3 (MMP-13) is a secreted matrix metalloproteinase encoded by the MMP13 gene, with a predicted molecular mass of 53.8 kDa and an observed ~54 kDa band in western blotting.
• It principally cleaves triple-helical fibrillar collagens, showing highest activity against soluble type II collagen, but also degrades types I, III, IV, X, and XIV, as well as fibronectin and tenascin.
• MMP13 belongs to the zinc- and calcium-dependent metalloprotease family; it requires calcium for stability and contains conserved domains typical of MMPs.
• The enzyme is secreted into the extracellular space and matrix, where it performs its catalytic functions.
• Expression is detected in fetal cartilage, hypertrophic chondrocytes, osteoblasts, periosteal cells, and activated T lymphocytes; it is also found in breast carcinoma tissue.
• Post-translational modifications include phosphorylation and glycosylation; the zymogen form contains a signal peptide and conserved disulfide bonds.
• MMP13 is essential for normal embryonic bone development, skeleton ossification, and bone fracture healing; it also promotes keratinocyte migration in wound healing and may facilitate tumor cell invasion.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to residues 350–450 of human MMP13, which lies in a region likely conserved across the active enzyme.
• For western blotting, a single band near 54 kDa is typically observed under reducing conditions; validate expression in appropriate cell lines or tissue lysates.
• In immunofluorescence/ICC, signal is expected in the extracellular matrix and secretory pathway; consider co-staining with organelle markers.
• ELISA applications may require pairing with a suitable capture antibody; test linearity using recombinant MMP13 standards.
• Since MMP13 is a secreted protein, cell culture supernatants or tissue extracts are suitable sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and research labs with comprehensive access to high-quality IVD raw materials. This MMP13 monoclonal antibody exemplifies our portfolio for extracellular matrix and oncology-related targets. We offer validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and support for rare target sourcing—streamlining your path from concept to clinic.