Anti-MMP13 Monoclonal Antibody for WB, ELISA - P45452

Core Product Specifications and Parameters

Biological Background: MMP13 Function and Localization

• MMP13, also known as collagenase 3, is a member of the matrix metalloproteinase (MMP) family of zinc-dependent endopeptidases.
• It plays a crucial role in the degradation of extracellular matrix proteins, particularly fibrillar collagens such as type I, type II, and type III collagen.
• MMP13 cleaves triple helical collagens with highest activity towards soluble type II collagen, and also degrades collagen types IV, X, and XIV, fibronectin, and ACAN.
• Beyond matrix degradation, MMP13 can proteolytically activate growth factors like TGFB1 and degrade CCN2, influencing wound healing and tissue remodeling.
• Subcellularly, MMP13 is secreted into the extracellular space and matrix, where it functions in cartilage degradation, bone development, mineralization, and endochondral ossification.
• It is essential for normal embryonic bone development and bone fracture healing, and also contributes to keratinocyte migration during wound repair.
• MMP13 tissue expression is detected in fetal cartilage, calvaria, hypertrophic chondrocytes, osteoblasts, and periosteal cells; it is also found in T lymphocytes and breast carcinoma tissue.
• The enzyme is synthesized as an inactive zymogen (pro-MMP13) that requires proteolytic activation, and its activity is regulated by calcium and zinc ions.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 104-272 within the catalytic domain; consider verifying reactivity by Western blotting in relevant sample systems.
• For WB, a band around 54 kDa is expected; use appropriate loading controls and consider reducing conditions for optimal detection.
• For ELISA applications, validate with recombinant MMP13 or relevant tissue extracts to confirm sensitivity and specificity.
• Cross-reactivity with mouse is reported; suitable for mouse model studies but always validate in the experimental system of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this MMP13 monoclonal antibody as part of a comprehensive IVD raw-material portfolio supporting research into extracellular matrix biology, bone development, and tumor invasion. We offer one-stop access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. Partner with us to accelerate your diagnostic development from concept to clinic.