Anti-MMP12 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P39900

Core Product Specifications and Parameters

Biological Background: MMP12 Function and Localization

• Macrophage metalloelastase (MMP-12, also known as ME, HME, MMP12) is a secreted matrix metalloproteinase that belongs to the zinc-dependent endopeptidase family.
• MMP-12 exhibits significant elastolytic activity and is involved in tissue injury and remodeling, with a preference for leucine at the P1' site and aromatic/hydrophobic residues at P1.
• The enzyme is primarily expressed in alveolar macrophages and is not detected in peripheral blood monocytes, highlighting its role in lung tissue homeostasis and inflammation.
• Subcellularly, MMP-12 is secreted into the extracellular space and localizes to the extracellular matrix, where it degrades elastin and other matrix components.
• As a member of the MMP family, it contains characteristic domains including a pro-peptide that maintains latency (zymogen form), and requires calcium and zinc ions for activity.
• Structural features include disulfide bonds and glycosylation, as indicated by UniProt keywords; a 3D structure has been determined.
• The full-length protein has a predicted molecular weight of ~54 kDa (calculated 54002 Da), which corresponds to the band observed in Western blotting.

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide corresponding to residues 200-300 of human MMP-12 (NP_002417.2). When designing experiments, consider that this region is within the catalytic domain and may recognize both pro and active forms, but does not overlap with the signal peptide.
• For Western blotting, the predicted molecular weight is approximately 54 kDa; however, glycosylation may cause slight shifts in migration. Validate the observed band size in your specific sample system.
• In IF/ICC, MMP-12 is secreted, so permeabilization and fixation conditions should be optimized to retain extracellular matrix-associated signal. Co-staining with macrophage markers can help confirm specificity in tissue sections.
• For ELISA applications, recombinant MMP-12 or purified protein standards are recommended to establish standard curves and assess antibody binding efficiency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to IVD raw materials, including validated polyclonal antibodies like this MMP-12 Rabbit pAb for tissue remodeling and inflammation studies. Beyond single antibodies, we offer optimized antibody pairs, bulk ancillary reagents, and sourcing of rare-target raw materials, streamlining your development from discovery to clinical application.