Anti-MMP11 Rabbit Polyclonal Antibody for WB, ELISA - P24347

Core Product Specifications and Parameters

Biological Background: MMP11 Function and Localization

• MMP11, also referred to as stromelysin-3 (alternatively: ST3, SL-3, STMY3), is a 55 kDa member of the matrix metalloproteinase family encoded by the MMP11 gene on human chromosome 22.
• According to UniProt, MMP11 may play an important role in the progression of epithelial malignancies, particularly through its activity in the tumor microenvironment.
• Classified as a calcium- and zinc-dependent metalloprotease, MMP11 features conserved structural domains including a signal peptide, propeptide, catalytic domain, and hemopexin-like repeats, which are typical of secreted MMPs.
• The protein is synthesized as a zymogen that requires proteolytic cleavage for activation, and its sequence contains multiple disulfide bonds essential for structural stability.
• MMP11 is localized to the extracellular matrix after secretion, consistent with its putative involvement in extracellular matrix remodeling and collagen degradation.
• Tissue-specific expression analysis reveals that MMP11 is predominantly found in stromal cells of breast carcinomas, underscoring its relevance in cancer biomarker studies.

Experimental Guidance and Technical Tips

• The antibody was generated against a recombinant protein encompassing amino acids 239-488 of human MMP11, which includes the hemopexin-like domains; this broad immunogen design may yield a polyclonal antibody with diverse epitope recognition, enhancing detection sensitivity across sample types.
• For Western blot, a specific band at approximately 55 kDa is expected; include appropriate loading controls and validate expression in tissue panels containing breast carcinoma specimens, where MMP11 is highly expressed.
• In ELISA, because polyclonal antibodies can cross-react with conserved epitopes within the MMP family, consider testing against other MMPs if assay specificity is critical.
• Upon arrival (shipped on ice bags), aliquot the antibody and store at -20°C for short-term use or -80°C for long-term storage; avoid repeated freeze-thaw cycles to maintain stability.

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CamelBio serves as a comprehensive procurement partner for diagnostic manufacturers and research entities, providing high-quality IVD raw materials including validated antibody pairs, optimized polyclonal antibodies such as this anti-MMP11 product, and ancillary reagents for cancer research applications. Whether your project requires bulk quantities, custom sourcing for rare targets, or technical consulting, our platform streamlines the transition from concept to clinical development, backed by rigorous supply chain management.