Anti-MMP10 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P09238

Core Product Specifications and Parameters

Biological Background: MMP10 Function and Localization

• MMP10 (Stromelysin-2) is also known as matrix metalloproteinase-10 (MMP-10) or Transin-2, encoded by the MMP10 gene.
• It is a secreted zinc-dependent endopeptidase that degrades various extracellular matrix components, including fibronectin, gelatins of type I, III, IV, and V, and weakly collagens III, IV, and V.
• MMP10 can activate procollagenase, thereby participating in the proteolytic cascade that remodels the extracellular matrix.
• As a member of the matrix metalloproteinase family, it shares structural features such as a signal peptide, propeptide domain, and catalytic domain with conserved zinc-binding and calcium-binding sites.
• Subcellularly, MMP10 is secreted into the extracellular space and localizes to the extracellular matrix, consistent with its role in matrix turnover.
• The enzyme is synthesized as an inactive zymogen and requires proteolytic cleavage for activation, a regulatory mechanism typical of MMPs.
• Post-translational modifications include disulfide bond formation, and the protein contains characteristic repeat structures and metal-binding motifs essential for its hydrolytic activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the mature protein (amino acids 237–476), which may recognize both the pro-form and active enzyme without preference. Validate in your model system if active-form-specific detection is needed.
• For Western blotting, expect a band near 54 kDa (precursor) or slightly lower for the mature form after activation; reducing conditions are recommended.
• In immunohistochemistry (IHC-P), antigen retrieval using heat-mediated citrate or EDTA buffer may enhance signal; tissue expression levels can be variable, so appropriate controls are essential.
• For immunofluorescence/immunocytochemistry (IF/ICC), fixation and permeabilization protocols should be optimized, as MMP10 is secreted and may require a Golgi-blocking agent to enhance intracellular retention.
• When used in ELISA, the polyclonal nature can provide sensitive capture/detection, but cross-reactivity with other MMPs should be assessed given sequence homology within the family.
• As a polyclonal antibody, lot-to-lot consistency may vary; it is advisable to titrate each new lot and confirm reactivity across intended species (human, mouse, rat).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, from validated antibody pairs and monoclonal/polyclonal antibodies to bulk ancillary reagents. Our MMP10 antibody offering serves as a robust component for developing assays in extracellular matrix remodeling, cancer progression, and inflammatory disease research. Leveraging our one-stop sourcing model, we can also fulfill custom requirements for rare targets or specialized reagent formats, helping you streamline your product development workflow from concept to clinic.