Anti-MMACHC Rabbit Polyclonal Antibody for WB, ELISA - Q9Y4U1

Core Product Specifications and Parameters

Biological Background: MMACHC Function and Localization

• MMACHC (cblC) is a cytosolic cobalamin chaperone and enzyme with dual activities: cyanocobalamin reductase (cyanide elimination) and alkylcobalamin dealkylase (glutathione transferase).
• It catalyzes the reductive decyanation of cyanocobalamin (inactive dietary B12) to cob(II)alamin using FAD/FMN and NADPH, a critical step for producing active cofactors methylcobalamin and adenosylcobalamin. Related references: PMID:18779575, PMID:19700356, PMID:21697092
• MMACHC also functions as a glutathione-dependent dealkylase, converting methylcobalamin and adenosylcobalamin to cob(I)alamin, enabling cellular interconversion of cofactors. Related references: PMID:19801555, PMID:21697092, PMID:22642810
• It forms a transport complex with ABCD4 and LMBRD1 to mediate lysosomal export of cobalamin into the cytosol. Related references: PMID:25535791
• In the cytosol, MMACHC assembles into a multiprotein complex with MMADHC, MTRR, and MTR to safely shuttle cobalamin to methionine synthase. Related references: PMID:21071249, PMID:27771510
• The protein is widely expressed, with highest levels in fetal liver and lymphoid tissues; it is weakly expressed in peripheral blood leukocytes.
• Keywords include FAD, FMN, NADP binding; cobalt and cobalamin cofactor handling; and association with methylmalonic aciduria and homocystinuria type C disease variants.
• UniProt notes a molecular weight of 31.7 kDa, consistent with the 32 kDa observed band.

Experimental Guidance and Technical Tips

• The immunogen (aa 63-282) covers the core catalytic domain; cross-reactivity with mouse and rat is reported, but validate in your specific sample system.
• For WB, expect a band near 32 kDa; inclusion of positive control (e.g., HeLa or liver lysate) is recommended.
• For ELISA, the polyclonal nature may provide robust signal; consider direct or sandwich ELISA formats depending on assay requirements.
• Optimize primary antibody dilution empirically, as performance may vary with sample type and experimental conditions.
• Note that MMACHC is widely expressed but may be low in leukocytes; choose tissue/cell lines accordingly.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio offers one-stop sourcing for validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our supply network covers rare targets like MMACHC, supporting cobalamin metabolism research and diagnostic development. From discovery to clinical application, CamelBio bridges your project needs with reliable, scalable raw materials.