Anti-MLYCD Polyclonal Antibody for WB, ELISA - O95822

Core Product Specifications and Parameters

Biological Background: MLYCD Function and Localization

• MLYCD encodes malonyl-CoA decarboxylase, a mitochondrial enzyme that catalyzes the conversion of malonyl-CoA to acetyl-CoA, playing a central role in fatty acid metabolism.
• In fatty acid biosynthesis, MLYCD selectively removes malonyl-CoA, ensuring that methyl-malonyl-CoA is the sole chain-elongating substrate for fatty acid synthase, leading to the production of fatty acids with multiple methyl side chains.
• Within peroxisomes, MLYCD is involved in degrading intraperoxisomal malonyl-CoA generated during the beta-oxidation of odd-chain-length dicarboxylic fatty acids.
• MLYCD contributes to the metabolic balance between glucose and lipid oxidation in muscle, acting independently of insulin signaling alterations, and may help control ischemic injury by enhancing glucose oxidation.
• Subcellularly, MLYCD localizes to the cytoplasm, mitochondrial matrix, and peroxisome matrix, reflecting its multi-organellar metabolic functions.
• Tissue expression is highest in heart, liver, skeletal muscle, kidney, and pancreas, with weaker expression in brain, placenta, spleen, thymus, testis, ovary, and small intestine; protein expression is confirmed in fibroblasts and hepatoblastoma cells.

Experimental Guidance and Technical Tips

• For western blot, consider titrating the antibody and using positive controls from tissues with high MLYCD expression, such as heart or liver lysates, to assess specificity.
• Given the immunogen covers amino acids 40–190, the antibody may detect both the full-length protein (~55 kDa) and any isoforms; confirm band patterns against expected molecular weights.
• In ELISA applications, ensure the coating antigen matches the immunogen sequence or purified MLYCD protein; paired detection antibodies may be required for sandwich ELISA development.
• Since cross-reactivity with mouse and rat is indicated, the antibody can be used for comparative studies, but validation in the relevant species’ sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to provide a comprehensive portfolio of IVD raw materials, including this anti-MLYCD polyclonal antibody suited for metabolic and mitochondrial research. Our sourcing capabilities extend to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target procurement to support your assay development from concept to clinic. For reliable access to high-quality reagents in fatty acid metabolism studies, trust CamelBio as your integrated supply partner.