Anti-MLST8 Rabbit Polyclonal Antibody for WB and ELISA - Q9BVC4

Core Product Specifications and Parameters

Biological Background: MLST8 Function and Localization

• MLST8 is a subunit of both mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals. Related references: PMID:12718876 PMID:15268862 PMID:15467718

• As part of mTORC1, MLST8 directly interacts with MTOR to enhance its kinase activity and, in nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction to promote RPTOR-mediated inhibition. mTORC1 promotes protein, lipid, and nucleotide synthesis while inhibiting autophagy through phosphorylation of ULK1, ATG13, and TFEB. Related references: PMID:12718876 PMID:15268862 PMID:15467718 PMID:24403073

• As part of mTORC2, MLST8 transduces growth factor signals to regulate proliferation, cytoskeletal organization, lipogenesis, and anabolic output. mTORC2 phosphorylates AKT, PKC, and SGK1, and promotes actin stress fiber formation. MLST8 acts as a bridge between MAPKAP1/SIN1 and MTOR within mTORC2. Related references: PMID:15467718 PMID:35926713 PMID:31085701

• MLST8 localizes primarily to the lysosome membrane and cytoplasm, consistent with its role in mTORC1 lysosomal recruitment and signaling.

• MLST8 is broadly expressed, with highest levels in skeletal muscle, heart, and kidney.

• MLST8 contains WD repeat domains and undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitin-like conjugation. Alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human MLST8 (aa 1-326), suggesting the antibody may recognize multiple epitopes. In WB, a band near 36 kDa is expected; consider using whole cell lysates or immunoprecipitated samples.
• For ELISA, the antibody can serve as a capture or detection reagent; validate with recombinant MLST8 protein to ensure specificity.
• This antibody cross-reacts with mouse and rat MLST8, making it suitable for rodent model studies; verify reactivity in your specific sample matrix.
• As a KD-validated antibody, it has been tested in knockdown samples; include appropriate positive and negative controls in your experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a one-stop sourcing solution for IVD raw materials, including validated antibodies for key signaling targets like MLST8. Whether you need optimized polyclonal antibodies, antibody pairs, or bulk ancillary reagents, our supply chain ensures efficient procurement from concept to clinic. Explore our mTOR pathway portfolio to support your assay development.