Anti-MLST8 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9BVC4

Core Product Specifications and Parameters

Biological Background: MLST8 Function and Localization

• MLST8 (also known as GbetaL, mLST8) is a core subunit of both mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2), essential regulators of cell growth and survival. Related references: PMID:12718876 PMID:15268862 PMID:15467718

• In mTORC1, MLST8 directly binds mTOR and enhances its kinase activity, promoting anabolic processes such as protein, lipid, and nucleotide synthesis; under nutrient-poor conditions, it stabilizes the RPTOR-MTOR interaction to inhibit mTOR activity. Related references: PMID:12718876

• mTORC2, which contains MLST8, is activated by growth factors and regulates cell proliferation, cytoskeletal organization, and survival via phosphorylation of AGC kinases (AKT, PKC, SGK1). Related references: PMID:15467718 PMID:35926713

• Within mTORC2, MLST8 acts as a bridge between MAPKAP1/SIN1 and MTOR, facilitating signal transduction. Related references: PMID:31085701

• The protein localizes to the lysosome membrane and cytoplasm, consistent with its role in nutrient sensing and signaling.

• MLST8 is broadly expressed, with highest levels in skeletal muscle, heart, and kidney.

• MLST8 contains WD repeat domains and is subject to post-translational modifications including acetylation, phosphorylation, and ubiquitination.

• The MLST8 gene produces alternatively spliced isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–326) of human MLST8; this region is conserved across isoforms, so the antibody may recognize multiple splice variants.
• For Western blotting, expect a band at approximately 36 kDa; consider using reducing conditions and validate in the relevant cell or tissue lysate.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), endogenous MLST8 may require appropriate fixation and permeabilization; a negative control (e.g., blocking peptide or knockout cell line) is recommended.
• Given cross-reactivity with mouse and rat, this antibody is suitable for rodent models; confirm specificity in the relevant species via knockdown or knockout controls.
• For ELISA, consider testing a range of antibody dilutions against recombinant MLST8 protein or peptide to establish optimal assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

As a leading IVD raw-material partner, CamelBio streamlines your procurement from concept to clinic, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. The MLST8 antibody area addresses mTOR signaling research, critical for cancer and metabolic disorders, and CamelBio can source rare-target raw materials and provide technical consulting to accelerate your diagnostic development. We ensure reliable supply and consistent quality to support your IVD pipeline.