Anti-MLPH Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9BV36

Core Product Specifications and Parameters

Biological Background: Melanophilin Function and Localization

• Melanophilin (MLPH), also known as Exophilin-3 or SlaC2-a, is a Rab effector protein encoded by the MLPH gene in humans.
• It functions as a crucial link in melanosome transport, connecting melanosome-bound RAB27A to the motor protein MYO5A.
• MLPH belongs to the synaptotagmin-like protein family and contains characteristic coiled-coil and zinc-finger domains.
• Subcellularly, MLPH is localized to the cytoplasm, where it facilitates intracellular trafficking processes.
• The protein undergoes alternative splicing, producing multiple isoforms that may have distinct roles.
• MLPH is associated with disease variants; mutations in this gene are linked to pigmentation disorders (e.g., Griscelli syndrome type 3).
• It is a metal-binding protein with zinc-coordinating motifs and contains repeat regions.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band near 66 kDa; validate detection in human, mouse, or rat lysates.
• In IHC-P, antigen retrieval may be necessary; optimize primary antibody concentration and detection system.
• For IF/ICC, suitable fixation and permeabilization should be tested; co-staining with melanosome markers can confirm specificity.
• The antibody can be used in ELISA; consider checkerboard titration to determine optimal coating and antibody concentrations.
• The immunogen corresponds to amino acids 301-600 of human MLPH; verify reactivity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with seamless access to IVD raw materials, technical services, and consulting for melanosome biology and related areas. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to support your workflow from concept to clinic. Let CamelBio be your reliable partner in advancing melanosome transport research and diagnostic development.