Anti-MLN Polyclonal Antibody for WB, IHC-P, ELISA - P12872

Core Product Specifications and Parameters

Biological Background: MLN Function and Localization

• MLN encodes promotilin, a precursor protein that is proteolytically processed to yield the active peptide hormone motilin.
• Motilin is secreted from endocrine cells of the gastrointestinal tract and plays a critical role in regulating interdigestive gastrointestinal motility.
• It indirectly stimulates rhythmic contraction of duodenal and colonic smooth muscle, facilitating coordinated peristalsis during fasting.
• The protein features a signal peptide and undergoes cleavage at a pair of basic residues to release the mature motilin hormone.
• UniProt keywords highlight its classification as a secreted hormone, with documented 3D structure, alternative splicing, and direct protein sequencing evidence.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 26–115, covering the mature motilin peptide region; consider validating antibody reactivity against both full-length and processed motilin in relevant samples.
• For Western blot, the predicted molecular weight of full-length promotilin is ~13 kDa; use appropriate gastrointestinal tissue lysates as positive controls and confirm detection of processed forms if expected.
• For IHC-P, human duodenum or colon sections serve as suitable positive tissues; optimize antigen retrieval and primary antibody dilution for your specific protocol.
• Cross-reactivity with mouse enables translational studies; ensure species-specific validation by comparing detection patterns in mouse gastrointestinal tissues.
• ELISA applications may require pairing with a suitable capture or detection antibody; assess performance using recombinant motilin or peptide fragments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-MLN antibody supports research into gastrointestinal motility and hormone signaling pathways. From concept to clinic, CamelBio delivers tailored procurement and supply solutions to accelerate your assay development.