Anti-MLLT3/AF9 Rabbit Polyclonal Antibody for WB and ELISA - P42568

Core Product Specifications and Parameters

Biological Background: MLLT3/AF9 Function and Localization

• MLLT3/AF9 functions as a chromatin reader in the super elongation complex (SEC), which increases the catalytic rate of RNA polymerase II transcription by suppressing transient pausing at multiple sites along the DNA. Related references: PMID:20159561 PMID:20471948 PMID:25417107

• The protein specifically recognizes and binds acylated histone H3, with highest affinity for crotonylated H3 at Lys-9 (H3K9cr) and Lys-18 (H3K18cr); it also binds acetylated and butyrylated H3K9 with lower affinity. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Related references: PMID:25417107 PMID:27105114 PMID:27545619 PMID:30385749

• Within the SEC, MLLT3/AF9 recruits the complex to crotonylated histones, promoting the recruitment of DOT1L and the deposition of histone H3 Lys-79 methylation (H3K79me), a histone mark associated with active transcription. Related references: PMID:25417107 PMID:27105114 PMID:27545619

• It plays a key role in hematopoietic stem cell (HSC) maintenance by preserving stemness, binding to transcription start sites of active genes in HSCs and sustaining H3K79me2 levels through DOT1L. Related references: PMID:31776511

• MLLT3/AF9 may also be involved in leukemogenic gene transcription, as suggested by recent studies. Related references: PMID:39794553

• The protein localizes to the nucleus, associating with chromosomes to exert its regulatory functions on chromatin.

• It is enriched in undifferentiated hematopoietic stem cells from fetal liver, cord blood, and bone marrow, consistent with its role in HSC biology.

• Post-translational modifications include phosphorylation and ubiquitin-like conjugation, as identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–150 of human MLLT3/AF9, encompassing the N-terminal region that includes the YEATS domain known for acylated histone binding. This design may favor detection of full-length MLLT3/AF9 and potentially some splice isoforms.
• This rabbit polyclonal antibody is suitable for western blot (WB) and ELISA applications, as validated on human samples. For WB, consider using whole-cell lysates from human cell lines expressing MLLT3/AF9, such as hematopoietic cells; the predicted molecular weight is ~63 kDa.
• For ELISA, the antibody can be used as either capture or detection reagent after appropriate pairing tests. Cross-reactivity with other proteins has not been extensively tested; validate in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This MLLT3/AF9 rabbit polyclonal antibody supports epigenetics and leukemia research, and CamelBio can assist with sourcing rare targets and customization to accelerate your assay development from concept to clinic.