Anti-MLLT3/AF9 Rabbit Monoclonal Antibody for WB, ELISA - P42568

Core Product Specifications and Parameters

Biological Background: MLLT3/AF9 Function and Localization

• MLLT3/AF9 (Protein AF-9, YEATS3) is a chromatin reader that specifically binds acylated histone H3, preferentially recognizing crotonylation marks that decorate active promoters and enhancers. Related references: PMID:25417107 PMID:27105114 PMID:27545619
• As a core component of the super elongation complex (SEC), it enhances RNA polymerase II processivity by suppressing transient pausing and recruits DOT1L to deposit H3K79 methylation, thereby modulating active chromatin states. Related references: PMID:20159561 PMID:20471948 PMID:25417107 PMID:27105114 PMID:27545619
• It is essential for hematopoietic stem cell (HSC) maintenance by preserving stemness, binding near transcription start sites in HSCs to sustain H3K79me2 levels via DOT1L recruitment. Related references: PMID:31776511
• May contribute to leukemogenic gene transcription through its role in aberrant H3K79 hypermethylation and gene activation. Related references: PMID:39794553
• Localizes to the nucleus and associates with chromosomes, consistent with its chromatin reader and transcriptional regulatory functions.
• Enriched in undifferentiated hematopoietic stem cells from fetal liver, cord blood, and bone marrow.
• Classified as a proto-oncogene and is frequently involved in chromosomal rearrangements in acute leukemia; also subject to phosphorylation and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-150) of MLLT3/AF9; consider this when validating detection of full-length and potential isoform variants.
• For Western blot, an expected band around 63 kDa (human) should be observed; results may vary based on post-translational modifications such as phosphorylation.
• Validate the antibody in human hematopoietic cell lines or cancer cell lines where MLLT3/AF9 is expressed; cross-reactivity with other species has not been tested.
• In ELISA applications, optimize coating conditions and detection parameters; the antibody's reactivity with recombinant immunogen suggests suitability for capture/detection but requires empirical validation.

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