Anti-MLLT1/ENL Polyclonal Antibody for WB and ELISA - Q03111

Core Product Specifications and Parameters

Biological Background: MLLT1/ENL Function and Localization

• ENL (MLLT1) is a YEATS domain-containing chromatin reader and an essential component of the super elongation complex (SEC), which enhances RNA polymerase II transcription by reducing promoter-proximal pausing. Related references: PMID:20159561, PMID:20471948
• The YEATS domain of ENL preferentially binds crotonylated histones, with a higher affinity for histone H3 crotonylated at lysine 27 (H3K27cr) compared to lysine 20 (H3K9cr20). Related references: PMID:27105114
• In acute myeloid leukemia (AML), ENL acts as a critical chromatin reader by recognizing acetylated histones, promoting oncogenic gene expression and leukemogenesis. Related references: PMID:39794553
• ENL localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.
• The protein undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitin-like conjugation, which may modulate its chromatin-binding and transcriptional activities.
• ENL is implicated in chromosomal rearrangements such as the t(11;19) translocation, leading to fusion proteins associated with mixed lineage leukemias.
• The molecular weight of human ENL is approximately 62 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 190–270 of human MLLT1, a region possibly within the conserved YEATS domain; cross-reactivity with mouse and rat suggests sequence conservation, but alignments should be checked when extending to other species.
• For western blotting, a band near 62 kDa is expected; validate with appropriate positive and negative cell or tissue lysates, and consider using nuclear extracts to enrich the target.
• ELISA optimization may require titration of both coating antigen and antibody; inclusion of appropriate blocking and wash steps can help minimize background.
• Given the role of ENL in leukemia, consider relevant cellular models (e.g., AML cell lines) for functional validation, but always confirm expression and specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, providing diagnostic manufacturers and research institutions with streamlined access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For chromatin and transcription targets like ENL, CamelBio can support your procurement needs—whether you require this polyclonal antibody for WB/ELISA, or assistance sourcing rare raw materials and developing custom immunoassays. Our integrated supply chain and technical consulting aim to accelerate your projects from concept to clinic.