Anti-MLL5/KMT2E Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q8IZD2

Core Product Specifications and Parameters

Biological Background: MLL5/KMT2E Function and Localization

• MLL5, also known as histone reader KMT2E, is an inactive histone-lysine N-methyltransferase 2E that acts as a chromatin-associated regulator. It binds tri-methylated histone H3 at Lys-4 (H3K4me3) via its PHD finger domain, mediating chromatin interaction downstream of transcriptional start sites of active genes. Related references: PMID:23798402 PMID:24130829

• It plays a key role in hematopoiesis, regulating terminal myeloid differentiation and hematopoietic stem cell self-renewal through a mechanism involving DNA methylation (By similarity).

• MLL5 participates in cell cycle regulation at multiple stages, including G1/S transition, S phase progression, and mitotic entry. It is recruited to E2F1-responsive promoters by HCFC1, where it stimulates H3K4me3 and transcriptional activation, facilitating G1 to S phase transition. Related references: PMID:14718661 PMID:18573682 PMID:19264965 PMID:23629655

• Isoform NKp44L functions as a cellular ligand for the natural cytotoxicity receptor NCR2/NKp44, potentially acting as a danger signal in NK-cell-mediated innate immunity.

• Subcellularly, MLL5 localizes to the nucleus (nucleoplasm and nuclear speckles), but can also be found in the cytoplasm, centrosome, and cell membrane depending on the isoform and cellular context.

• The protein is widely expressed in both adult and fetal tissues, with highest levels in fetal thymus and kidney, and adult hematopoietic tissues, jejunum, and cerebellum. Isoform NKp44L is not detected on healthy circulating cells but is expressed on many tumor and transformed cell lines. Related references: PMID:12101424 PMID:23958951

• Structurally, MLL5 features a SET domain (inactive methyltransferase), PHD finger, and coiled-coil regions, and is subject to post-translational modifications including phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 200-500, a region containing the PHD finger domain. This antibody may be particularly suitable for detecting chromatin-bound MLL5 in ChIP-related applications if adapted.
• For Western blot, the predicted molecular weight is ~205 kDa; consider using a low-percentage gel and extended transfer times. Validate specificity with appropriate controls, as multiple isoforms exist.
• In immunofluorescence and immunohistochemistry, MLL5 can display nuclear speckle or centrosomal staining patterns depending on cell type and fixation. Include permeabilization steps and co-staining with organelle markers.
• For ELISA, use the recombinant protein immunogen as a positive coating antigen to establish assay conditions. Cross-reactivity with mouse and rat MLL5 may allow multi-species testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials and technical support—from early-stage development to clinical application. For MLL5/KMT2E-focused projects in hematology, oncology, or chromatin biology, CamelBio offers validated antibody pairs, monoclonal/polyclonal antibody reagents, bulk ancillary supplies, and expertise in sourcing rare targets, streamlining your assay development and procurement needs.