Anti-MLKL Rabbit Polyclonal Antibody - Q8NB16

Core Product Specifications and Parameters

Biological Background: MLKL Function and Localization

• MLKL (Mixed lineage kinase domain-like protein) is a pseudokinase that plays a central role in TNF-induced necroptosis, a programmed cell death pathway.
  Related references: PMID:22265413, PMID:22265414, PMID:22421439
• Upon phosphorylation by RIPK3, MLKL homotrimerizes, translocates to the plasma membrane, and triggers necrotic death through calcium influx and membrane disruption.
  Related references: PMID:22265413, PMID:22265414, PMID:22421439
• In the nucleus, MLKL can mediate necroptosis in response to orthomyxovirus infection, leading to nuclear envelope disruption and cytosolic DNA leakage.
• MLKL binds inositol hexakisphosphate (InsP6), which is essential for its necroptotic function.
  Related references: PMID:29883610
• Subcellularly, the protein localizes to the cytoplasm, plasma membrane, and nucleus.
• MLKL is subject to alternative splicing and phosphorylation; it contains coiled-coil and nucleotide-binding regions but lacks catalytic kinase activity.

Experimental Guidance and Technical Tips

• For western blot detection of endogenous MLKL, consider preparing lysates from cells treated with necroptosis inducers (e.g., TNF + Smac mimetic + caspase inhibitor) and probe with the antibody at dilutions to be optimized.
• In immunofluorescence/immunocytochemistry, confirm membrane or cytoplasmic localization; permeabilization may be required for intracellular staining.
• For immunohistochemistry (IHC-P), antigen retrieval methods (e.g., heat-mediated citrate buffer) may improve signal; validate staining in known positive tissues.
• Cross-reactivity with mouse and rat has been reported, but performance should be verified in your specific sample matrix and protocol.
• The immunogen targets the C-terminal region (aa 369-471); this antibody may recognize multiple isoforms, but isoform specificity should be confirmed experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. For necroptosis and cell death research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies (including this MLKL pAb), bulk ancillary reagents, and customized sourcing for rare targets. From early-phase development to clinical application, our one‑stop supply chain simplifies your procurement of critical reagents.