Anti-MLKL Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9D2Y4

Core Product Specifications and Parameters

Biological Background: MLKL Function and Localization

• MLKL (mixed lineage kinase domain-like protein) is a pseudokinase that lacks intrinsic protein kinase activity. Related references: PMID:24012422
• It acts as a critical executor of TNF-induced necroptosis, becoming activated upon RIPK3-mediated phosphorylation, which triggers homotrimerization and translocation to the plasma membrane. Related references: PMID:23835476 PMID:24012422 PMID:24019532
• In response to orthomyxovirus infection, MLKL participates in nuclear necroptosis following ZBP1 sensing of Z-RNA; nuclear RIPK3 phosphorylates MLKL, leading to nuclear envelope disruption and cytosolic DNA leakage. Related references: PMID:32200799 PMID:32296175
• Binding to highly phosphorylated inositol phosphates such as InsP6 is essential for its necroptotic function.
• Subcellular localization spans the cytoplasm, cell membrane, and nucleus, reflecting its dynamic trafficking during cell death signaling.
• It is highly expressed in thymus, colon, intestine, liver, spleen, and lung, with lower levels in skeletal muscle, heart, and kidney; absent in brain.
• Structural features include a coiled-coil domain and a nucleotide-binding region, and it exists in multiple isoforms via alternative splicing.
• MLKL is regulated by phosphorylation, a key post-translational modification for its activation.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-53) of mouse MLKL, which may detect full-length and cleavage products; validate in your relevant sample system.
• For Western blot (WB), the predicted molecular weight is approximately 54 kDa; consider using reducing conditions and appropriate molecular weight markers.
• In immunostaining applications (IF-P, IHC-P), optimize permeabilization and antigen retrieval due to MLKL"s membrane and nuclear localization.
• For ELISA, this polyclonal antibody can serve as either capture or detection reagent; pairing with monoclonal antibodies may improve assay specificity.
• As a polyclonal antibody, lot-to-lot variability may occur; include appropriate controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research teams with end-to-end access to IVD raw materials, streamlining your journey from concept to clinic. This anti-MLKL antibody supports necroptosis and cell death research, and is just one example of our extensive portfolio covering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized raw-material sourcing for rare targets. Partner with us to secure reliable, performance-driven reagents tailored to your assay development needs.