Anti-MLH3 Monoclonal Antibody for WB, ELISA - Q9UHC1

Core Product Specifications and Parameters

Biological Background: MLH3 Function and Localization

• MLH3 (MutL homolog 3) is a DNA mismatch repair protein, also known as HNPCC7, that belongs to the MutL family.
• It is probably involved in the repair of DNA mismatches, contributing to the maintenance of genomic stability.
• The protein is ubiquitously expressed across tissues, consistent with its fundamental role in DNA repair.
• MLH3 localizes to the nucleus, where it functions in the DNA damage response.
• Mutations in MLH3 have been associated with hereditary nonpolyposis colorectal cancer (HNPCC), highlighting its critical role in tumor suppression.
• Alternative splicing of MLH3 generates multiple transcript variants, potentially modulating its function.
• The antibody's immunogen spans the C-terminal region (amino acids 1190–1389) of human MLH3, a region that may be important for protein interactions or regulatory functions.

Experimental Guidance and Technical Tips

• For Western blot, due to the large predicted molecular weight of MLH3 (164 kDa), consider using a low-percentage SDS-PAGE gel (e.g., 6–8%) and extend transfer time or use wet transfer for efficient detection.
• The antibody cross-reacts with both human and mouse MLH3; validate in the relevant species and tissue/cell line models for your experimental system.
• Since this is a recombinant monoclonal antibody, it offers high batch-to-batch consistency and reduced nonspecific binding compared to polyclonal antibodies; standard blocking and washing conditions are typically sufficient.
• For ELISA, direct or indirect coating of the immunogen or target protein may require optimization; the antibody is supplied as a liquid formulation, ready for dilution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with a comprehensive range of IVD raw materials, technical services, and consulting—from concept to clinic, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For targets like MLH3 involved in DNA repair and cancer diagnostics, CamelBio offers reliable sourcing for rare and specialized raw materials, supporting your assay development and manufacturing needs.