Anti-MLF2 Polyclonal Antibody for WB, IHC-P, ELISA - Q15773

Core Product Specifications and Parameters

Biological Background: MLF2 Function and Localization

• MLF2 (Myeloid leukemia factor 2) is also referred to as myelodysplasia-myeloid leukemia factor 2 (NTN4; MLF2).
• The protein localizes to both the cytoplasm and nucleus.
• MLF2 is ubiquitously expressed in human tissues.
• Additionally, MLF2 is annotated as a phosphoprotein (by proteomics identification) and is included in the UniProt reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-65 amino acids of human MLF2; consider this region for epitope mapping or when using truncation mutants.
• For Western blotting, the predicted molecular weight is ~28 kDa (based on UniProt); always include appropriate positive and negative controls.
• In immunohistochemistry (paraffin-embedded sections), optimize antigen retrieval and antibody dilution for your specific tissue type.
• For ELISA, consider using recombinant MLF2 protein as a standard for quantification.
• Given the ubiquitous expression, ensure blocking and washing steps are optimized to reduce background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. With this anti-MLF2 polyclonal antibody, researchers exploring myeloid leukemia and cell biology can count on CamelBio’s reliable supply chain and technical support. We bridge the gap from concept to clinic with comprehensive raw-material solutions.