Anti-MLF1 Polyclonal Antibody for WB, IF/ICC, ELISA - P58340

Core Product Specifications and Parameters

Biological Background: MLF1 Function and Localization

• MLF1 (Myeloid leukemia factor 1) is a protein involved in lineage commitment of primary hemopoietic progenitors, restricting erythroid formation while enhancing myeloid formation. It interferes with erythropoietin-induced erythroid terminal differentiation by preventing cell cycle exit via suppression of CDKN1B/p27Kip1 levels.
• MLF1 suppresses COP1 activity through CSN3, leading to p53 activation and induction of cell cycle arrest, thereby coupling differentiation control with tumor suppressor pathways.
• The protein binds DNA and influences the expression of multiple genes, suggesting a role as a transcription factor in the nucleus.
• MLF1 localizes to the cytoplasm, nucleus, cell projection cilium, and cytoskeleton (cilium basal body), indicating dynamic shuttling and participation in diverse cellular compartments.
• Tissue expression is highest in testis, ovary, skeletal muscle, heart, kidney, and colon, with low levels in spleen, thymus, and peripheral blood leukocytes, reflecting a broad but regulated tissue distribution.
• Post-translational modifications include phosphorylation, and the protein contains regions of alternative splicing, contributing to functional diversity. It is classified as a developmental protein and has been associated with chromosomal rearrangements in myeloid malignancies.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human MLF1 (amino acids 1-268), presented as a recombinant fusion protein. This design may confer broad epitope recognition suitable for detecting multiple isoforms and post-translationally modified forms.
• For Western blot (WB), a band at approximately 31 kDa is expected based on the calculated molecular weight. Validate the observed band size in the relevant species and sample type, as post-translational modifications or isoform variants may cause slight shifts.
• When using immunofluorescence/immunocytochemistry (IF/ICC), anticipate signals in both cytoplasmic and nuclear compartments, as well as potential localization to primary cilia, depending on cell type and culture conditions. Co-staining with organelle markers is recommended.
• The antibody has been shown to cross-react with mouse and rat, enabling comparative studies across these species. Nonetheless, confirm cross-reactivity in your specific experimental system, especially if using genetically modified models.
• For ELISA, consider pairing with a matched detection antibody if developing a sandwich assay; optimal coating and detection conditions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio provides diagnostic manufacturers and research institutes with streamlined access to high-quality antibodies, antigens, and ancillary reagents. This anti-MLF1 polyclonal antibody supports investigations into hematopoietic differentiation, cell cycle control, and leukemia biology—critical areas for developing novel diagnostic markers and therapeutic strategies. Beyond individual reagents, CamelBio offers comprehensive sourcing solutions including validated antibody pairs, bulk production options, and rare-target material procurement, helping you accelerate from concept to clinic with confidence.