Anti-MLD Monoclonal Antibody for WB, ELISA - O15121

Core Product Specifications and Parameters

Biological Background: MLD Function and Localization

• MLD, also known as sphingolipid delta(4)-desaturase DES1, is a transmembrane enzyme that introduces a double bond into sphingolipids, specifically converting D-erythro-sphinganine to D-erythro-sphingosine. Related references: PMID:11937514 PMID:30620337 PMID:30620338
• This reaction is a key step in the biosynthesis of sphingolipids, which are crucial for membrane structure and cell signaling.
• The enzyme also exhibits retinol isomerase activity, catalyzing the equilibrium isomerization of retinols, based on sequence similarity.
• MLD is localized to the mitochondrial membrane and endoplasmic reticulum membrane.
• It is ubiquitously expressed in human tissues.
• Post-translational modifications include phosphorylation, and genetic variants in the DEGS1 gene are associated with leukodystrophy.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-100 of human MLD; consider using this N-terminal region for epitope mapping or competitive assays.
• For WB, a band around 38 kDa is expected; optimize antibody dilution and validate in relevant human cell or tissue lysates.
• In ELISA, verify coating antigen integrity and use appropriate blocking steps to minimize background.
• Cross-reactivity with other species has not been validated; test in model organisms if intended for cross-species applications.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio supplies validated IVD raw materials, including monoclonal antibodies like this anti-MLD clone, to diagnostic developers and research labs worldwide.
• Beyond individual antibodies, we offer matched antibody pairs, bulk ancillary reagents, and sourcing for rare targets in lipid metabolism and neurodegenerative disease areas.
• Contact us to streamline your raw-material procurement from concept to clinic.