Anti-MKS1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NXB0

Core Product Specifications and Parameters

Biological Background: MKS1 Function and Localization

• MKS1 is also known as Meckel syndrome type 1 protein, encoded by the MKS1 gene, with alternative aliases including MES, MKS, BBS13, POC12, and JBTS28.
• It is a core component of the tectonic-like complex, which localizes to the transition zone of primary cilia and acts as a barrier preventing free diffusion of transmembrane proteins between the cilia and plasma membranes.
• MKS1 is essential for ciliogenesis, playing a role in centrosome migration to the apical cell surface during early cilium formation.
• The protein is required for maintaining ciliary structure and function, contributing to the regulation of ciliary length and number through modulation of centrosome duplication.
• Subcellularly, MKS1 is found in the cytoplasm, associated with the cytoskeleton, at the cilium basal body, and at the centrosome/microtubule organizing center.
• Mutations in MKS1 are linked to several ciliopathies, including Meckel syndrome, Bardet-Biedl syndrome, and Joubert syndrome, underscoring its critical role in cilium biogenesis and degradation.
• The MKS1 protein may undergo alternative splicing, as indicated by UniProt annotations, with a reference molecular weight of approximately 65 kDa (calculated 64528 Da).

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a recombinant fusion protein corresponding to amino acids 150-300 of human MKS1. This central region may avoid highly conserved domains, potentially contributing to species cross-reactivity with mouse and rat.
• Western blot application: A band around 65 kDa is expected; consider validating in ciliated cell lines, as MKS1 expression may correlate with ciliary presence.
• Immunofluorescence: Given the localization to centrosomes and basal bodies, IF/ICC may reveal punctate staining at the base of cilia. Use appropriate fixation and permeabilization methods.
• ELISA application: The recombinant immunogen can serve as a positive control for sandwich ELISA development; consider pairing with a validated capture antibody for quantitative studies.
• Cross-reactivity: This antibody has been shown to react with human, mouse, and rat, making it suitable for cross-species studies; however, users should validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-MKS1 antibody supports research into ciliary biology and associated diseases. Our portfolio includes validated antibody pairs and ancillary reagents to accelerate your diagnostic development and research needs.