Anti-MKRN3 Rabbit Polyclonal Antibody for WB and ELISA - Q13064

Core Product Specifications and Parameters

Biological Background: MKRN3 Function and Localization

• MKRN3 is a RING finger E3 ubiquitin ligase, also known as RNF63, ZNF127, and CPPB2, playing a critical role in reproductive timing.
• The protein functions as a key developmental timer by inhibiting premature activation of the reproductive hormone cascade, specifically regulating gonadotropin-releasing hormone (GNRH1) expression and mRNA stability.
• MKRN3 epigenetically silences GNRH1 transcription by disrupting MBD3 binding to the GNRH1 promoter via non-proteolytic 'Lys27'-linked ubiquitination of MBD3, thereby modulating regional methylation. Related references: PMID:34692086
• It also modulates GNRH1 mRNA translation and stability through non-proteolytic ubiquitination of PABP family members (PABPC1, PABPC3, PABPC4) at multiple sites. Related references: PMID:33744966
• Additionally, MKRN3 maintains genomic and epigenomic stability by regulating APEX2 abundance via 'Lys-48'-linked ubiquitination, targeting it for proteasomal degradation. Related references: PMID:38705397
• The protein localizes predominantly to the nucleus, consistent with its regulatory roles in transcription and epigenetic modulation.
• MKRN3 is ubiquitously expressed across tissues, indicating a broad regulatory function beyond neuroendocrine control.
• It contains characteristic zinc-finger domains and a RING finger motif, classifying it as a metal-binding transferase in the ubiquitin conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-260) of human MKRN3, potentially allowing detection of full-length and N-terminal isoforms; validate specificity in your sample system.
• For Western blot, expect a band around 56 kDa; consider using molecular weight markers and confirm identity with knockdown or knockout controls.
• This antibody is reported to cross-react with mouse MKRN3, enabling its use in mouse models; verify cross-reactivity under your experimental conditions.
• For ELISA, optimize the antibody dilution and antigen coating conditions through a checkerboard titration to achieve the best signal-to-noise ratio.
• The antibody is suitable for detecting both proteolytic and non-proteolytic ubiquitination events involving MKRN3; consider co-IP or ubiquitin-specific assays to study these interactions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For reproductive health and neuroendocrine research, our supply network can source rare-target antibodies like anti-MKRN3, backed by technical consultation from concept to clinic. Contact us to customize your antibody sourcing needs.